Studies on the Extraction of DNA from Human: Resourceful Material for the Identification
Ahmed Kareem Hussein Alatafi *
Department of Biology, College of Science, University of Diyala, Iraq.
Noor Hassan Jumaa
Department of Biotechnology, College of Science, University of Diyala, Iraq.
Mariam Hussein Ali
Department of Biotechnology, College of Science, University of Diyala, Iraq.
Zahraa Abd Alamir Ali
Department of Biotechnology, College of Science, University of Diyala, Iraq.
Zainab Zanzal Ahmed
Department of Biotechnology, College of Science, University of Diyala, Iraq.
*Author to whom correspondence should be addressed.
Abstract
The extraction of DNA from human samples is a pivotal process in forensic science, medicine, and genetics, serving as a resourceful tool for identification purposes. This study explores various methods and techniques employed in DNA extraction from human tissues, focusing on their efficiency, reliability, and applicability in different contexts. Additionally, the impact of sample quality, degradation, and contamination on DNA yield and integrity is discussed. We compared 5 different methods to isolate DNA from human saliva and cigarette butts and result have been incorporated in form of gel images and UV-Spectrophotometer absorbance readings. Findings are represented in graphs for same sample with different methods.
Keywords: DNA, genetics, DNA extraction, forensic science, identification