Antioxidant Activity, Total Phenolic and Flavonoid Contents of Petroselinum crispum Mill.
Mortada Mohamed El-Sayed
Laboratory of Medicinal Chemistry, Theodor Bilharz Research Institute, Kornaish El-Nile-Warrak El-Hadar, Imbaba, Giza 12411, Egypt.
Nadia Hanafi Metwally
Department of Chemistry, Faculty of Science, Cairo University, Giza, Egypt.
Ibrahim Adel Ibrahim
Department of Chemistry, Faculty of Science, Cairo University, Giza, Egypt.
Heba Abdel-Hady
Laboratory of Medicinal Chemistry, Theodor Bilharz Research Institute, Kornaish El-Nile-Warrak El-Hadar, Imbaba, Giza 12411, Egypt.
Bassant Said Ahmed Abdel-Wahab *
Laboratory of Medicinal Chemistry, Theodor Bilharz Research Institute, Kornaish El-Nile-Warrak El-Hadar, Imbaba, Giza 12411, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Background: Petroselinum crispum is a common vegetable or spice in Egypt and worldwide. It possess many pharmacological and medicinal properties.
Aims: In the current research, the total phenolic and flavonoid contents as well as the antioxidant activities of P. crispum methanolic extract and its fractions were evaluated.
Methodology: The total phenolic content was estimated by Folin-Ciocalteu method and total flavonoid content was tested by aluminum chloride assay. The antioxidant activity was evaluated by 1, 1- diphenyl-2- picryl hydrazyl radical (DPPH) assay, 2, 2ʹ-azino- bis (3-ethylbenzthiazoline-6-sulphonic acid) assay (ABTS), and total antioxidant capacity assay.
Results: The ethyl acetate fraction derived from the methanolic extract exhibited the highest total phenolic content (121.95±2.15, mg GAE/ g extract) and total flavonoids content (106.45±2.18 mg rutin equivalent / g extract). Furthermore, the ethyl acetate fraction demonstrated the more potent DPPH activity (Sc50= 44.51±2.06 µg/ml), ABTS activity (69.82±3.07 mmTrolox® eq. / 10 g extract) and total antioxidant capacity (166.83±1.96 mg of ascorbic acid eq. / g extract).
Conclusion: The results of the current study showed that P. crispum contains an important amount of phenolic compounds and higher antioxidant activities, which serve as a good source of food ingredients and dietary supplements.
Keywords: Petroselinum crispum, antioxidant properties, phenolics, DPPH assay.