In-Vitro Antimicrobial Effect of Different Honey Samples against Selected Micro-organisms Marketed in Abuja Nigeria
K. T. Olatunji *
Department of Microbiology, Human Virology and Biotechnology, National Institute for Pharmaceutical Research and Development, Idu, P.M.B. 21 Garki, Abuja, Nigeria.
M. Aboh
Department of Microbiology, Human Virology and Biotechnology, National Institute for Pharmaceutical Research and Development, Idu, P.M.B. 21 Garki, Abuja, Nigeria.
P. Oladosu
Department of Microbiology, Human Virology and Biotechnology, National Institute for Pharmaceutical Research and Development, Idu, P.M.B. 21 Garki, Abuja, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aim: This study aimed to investigate the antimicrobial effect of honey samples sold in different markets in Abuja, Nigeria against some selected microbial isolates.
Study Design: Five honey samples obtained from different markets in Abuja metropolis, Nigeria were used for this study against some medically important micro-organisms viz; Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella paratyphi, Klebsiella pneumonia and Candida albicans.
Place and Duration of Study: The study was conducted in Abuja, Nigeria at the Department of Microbiology and Biotechnology, National Institute for Pharmaceutical Research and Development [NIPRD], from January 2018 to February 2018.
Methodology: Agar diffusion technique of Microbiological standard was employed. Each of the 5 honey samples was diluted in two-fold dilutions to have 100, 50, 25 and 12.5% concentrations respectively and tested against the test organisms. A fixed-dose concentration of chloramphenicol at 30 µg/mL was used as a control drug against the bacterial isolates.
Results: All the honey samples showed varying degrees of inhibitory effect against all the test organisms. It inhibited all the organisms at 100% concentration while some samples showed activity at 50% concentration. However, only Salmonella paratyphi was susceptible at 25% while no effect was observed at 12.5% concentration for all the honey samples.
Conclusion: The study has shown that honey has an antimicrobial effect on certain micro-organisms, and could be used as an adjunct therapy against certain infections to minimise proliferation of multi-drug resistant micro-organisms in the society.
Keywords: Honey, antimicrobial, bacteria, resistance, susceptibility.