Anti-ulcer Mechanisms of L-lysine in Male Wistar Rats
Chukwu J. Okonkwo
Department of Physiology, College of Medicine, University of Ibadan, Oyo State, Nigeria
Francis S. Oluwole *
Department of Physiology, College of Medicine, University of Ibadan, Oyo State, Nigeria
Ayobami Dare
Department of Physiology, College of Health Sciences, Bingham University, Karu, Nasarawa State, Nigeria
*Author to whom correspondence should be addressed.
Abstract
L-lysine is an essential amino acid found in most protein food sources, in particular high-protein foods such as eggs, meat, soybean, milk and fish. This amino acid has been reported to have an indirect antioxidant property. Antioxidants are known to have gastroprotective property. This study examined the effect of L-lysine pre-treatment on indomethacin induced ulceration in male Wistar rats.
Fifty male Wistar rats were used for this study and were randomly divided into two study groups of twenty five (25) animals each. The first sub-group was used for the anti-ulcer studies; antioxidant enzymes (SOD, CAT, and MDA), Nitric oxide, parietal cell count, and the mean ulcer score, while the second sub-group was used for the gastric mucus secretion study. Each sub-group was divided into five groups with five animals per group namely: Control, Omeprazole (20 mg/kg), L-lysine (100 mg/kg, 200 mg/kg and 400 mg/kg).
The results showed that l-lysine pre-treatment significantly increased SOD activity and reduced MDA levels but with no significant change in catalase activity. NO levels in the treated groups were significantly higher than in the control. Gastric mucus secretion was significantly increased and the parietal cell count significantly reduced in L-lysine pre-treated animals.
The findings from this study reveal that l-lysine supplement has some anti-ulcer properties which might be mediated through increased antioxidant enzymes, increase gastric mucus secretion and inhibition of parietal cell synthesis. This will be beneficial in the treatment of peptic ulcer.
Keywords: Antioxidants, nitric oxide, omeprazole, L-lysine, anti-ulcers