An Investigation on Some Hepatic Enzymes and Haematological Variables among Alcoholic Volunteers in Kadima District of Jos South Local Government Area of Plateau State, Nigeria
Journal of Applied Life Sciences International,
Aim: This research work investigated the impact of the differences in duration of alcoholic beverage consumption on hepatic and heamatological parameters.
Methods: Fifty (50) healthy male volunteer subjects were recruited for this study from Kadima district of Jos South Local Government Area of Plateau State, Nigeria and were coded according to the years of alcohol consumption: 1-7, 8-14, 15-21, and 22-28 for groups B, C, D and E respectively with each having ten (10) volunteers. Group A was christened as the control having human subjects that have neither drank Burukutu nor factory- based lager beer. Full blood count was done using haematology analyser while spectrophometric method was used to assay for the activities of AST, ALT, ALP, GGT as well as various levels/concentrations of TB, DB, TP, urea and creatinine. Results: Result obtained showed that AST and ALP were raised (p < 0.05) in all the groups when compared to the control. ALT was elevated in group E only while GGT was increased (p < 0.05) in groups D and E. Total and direct bilirubin were both elevated (p < 0.05) in groups B, C and E but equal control in group D. Total protein was higher in groups B and C but lowered in groups D and E relative control. Concentrations of urea was statistically significant (p < 0.05) in groups C and E. Creatinine was only statistically significant in group C but equal control in other groups. Results of the haematological parameters showed that RBC and WBC were reduced in all the groups’ relative control. Hb concentration was increased (p < 0.05) in groups C, D and E. PCV was found to be reduced in groups B, C and D when compared to the control.
Conclusion: This study has shown that prolong ingestion of local brew impacts negatively on hepatic biomarkers as well as some haematological indexes like WBC, RBC and PCV.
- liver enzyme
How to Cite
Evans E. The oxford companion to beer. Oxford University Press. 2011;236.
Kolawole OM, Kayode MO, Akinduyo B. Proximate and microbial analyses of burukut and pito produced in Ilorin, Nigeria. Africa Journal of Biotechnology. 2007;6:587-590.
Kingsley CE, Victor E. EA kinetic study of burukutu fermentation. Journal of Engineering and Applied Sciences. 2007;2(7):1193-1198.
Ezeonu Chukwuma Stephen, Nwokwu Chukwumaobim Daniel U, Kadiri Bashir. Comparative physico-chemical analysis of locally brewed beer (Burukutu) from Corn, Millet and Sorghum. American Journal of Science and Technology. 2017;4(3):43-48
Egemba KC, Etuk VEA. Kinetic study of Burukutu fermentation. Journal of England Apple Science. 2007;2:1193-1198.
Odetokun SM. Practice guideline committee of the american association for the study of liver diseases and practice parameters committee of the American College of Gastroenterology. Mol. Nutr. Food Res. J. 41(6):375-377.
Lyumugabe F, Gros J, Nzungize J, Bajyana E, Thonart P. Characteristics of African traditional beers brewed with sorghum malt: a review. Biotech. Agro. Soc. Environ. 2012;16(4): 509- 530.
Ogunbanwo ST, Adewara, AO, Fowoyo PT. Effect of fermentation by pure cultures of Lactobacillus fermentum and Saccharomyces cerevisiae as starter cultures in the production of ‘burukutu’. New York Sci. J. 2013;6(1):73–81.
Adewara AO, Ogunbanwo ST. Effects of processing variables on the production of ‘burukutu’, a Nigerian fermented beverage. Nature Sci. 2013;11(1):16–28.
Gazuwa SY, Denkok Y. Organic Metabolites, Alcohol Content, and Microbial Contaminants in Sorghum-based Native Beers Consumed in Barkin Ladi Local Government Area, Nigeria. Journal of Scientific Research & Reports. 2017;17(4):1-8.
Gazuwa SY, Dabak JD, Ubom GA. Iron contamination of two local alcoholic drinks. Journal of Medicine in the Tropics. . 2006;8(1):32-38.
Bennet DR, Baird CJ, Chan K, Crookes PF, Bremmer CG, Gottlieb MM, Narikotu W. Zinc Toxicity following Massive Coin Ingestion. American Journal of Medicine and Pathology. 1997;18(2):143-153.
Halzelwood LA, Vanmaris AJ. Enrlich pathway for fused alcohol production. Appl Environ. Microbial. 2008;74:2259-2266.
Hassan MM, Hwang LY, Hatten CJ, Swaim M, Li D. Risk factors for hepatocellular carcinoma: Synergism of alcohol with viral hepatitis and diabetes mellitus. Hepatology. 2002;36:206-1213.
Purohit V, Gao B; Song BJ. Molecular mechanisms of alcoholic fatty liver. Alcohol. Clin. Exp. Res. 2009;33:191-205.
Pari L, Karthikesan K. Protective role of caffeic acid against alcohol-induced biochemical changes in rats. Fundaclin. Pharmacol. 2007;21:315-361.
McKillop IH, Schrum LW. Role of alcohol in liver carcinogenesis. Seminars in Liver Disease. 29: 222-232.
Becker U, Deis A, Sørensen TI, Grønbaek M, Borch-Johnsen K, Müller CF, Schnohr P, Jensen G. Prediction of risk of liver disease by alcohol intake, sex, and age: a prospective population study. Hepatology. 1996;23:1025-9.
Elanchezhian, Yoganandh T, Mayilsamy S, Shankar Radhakrishnan. Comparison of haematological parameters between alcoholics and non-alcoholics. International Journal of Research in Medical Sciences. 2017;5(11):5041-5047.
Osarentin A, Taiwo E, Chioma LA. Gender and alcohol consumption affect human serum enzymes, protein, bilirubin. Asian Journal of Biochemistry. 2007;2:330-336.
Stanley PC. Alcohol: A silent Killer. University of Port Harcourt Press Limited; 2003.
Mauro P, Renze B, Wouter P. In: Tietz Textbook of Clinical Chemistry and Molecular Diagnosis.(4th Edition).Carl AB, Edward R, David EB, editors. Elsevier, 2006, Enzymes; 604-616.
Alexandre L, Philippe M. Alcohol liver Disease: Mechanism of injury and targeted treatment, Nature Reviews Gastroenterology and Hepatology. 2015;12:231-242.
Klein RF, Fausti KA, Carlos AS. Ethanol inhibits human osteoblastic cell proliferation. Alcohol Clin Exp Res. 1996;20:572-578.
Uchendu Ikenna Kingsley, Chuntan Pius Jibrin, Agu Chidozie Elochukwu, Orji Oliver
Chukwuma, Chukwu John-Paul Ikechukwu, Nnedu Ebuka Bitrus, Okongwu Uzoma
Chinenye. Comparative Impact of Chronic Consumption of Burukutu and Beer on Liver Biomarkers in Male Volunteers in Akwanga Lga Narasawa State. Annals of Medical and Health Sciences Research. 2017;7(Special Issue 1);84-91.
Uchida T, Koa H, Quispe-Sjogren M, Peters RL. Alcoholic foamy degeneration: A pattern of acute alcoholic injury of the liver. Gastroenterology. 1983;84:683- 692.
Nalpas B, Vassault A, Le Guillou A, Lesgourgues B, Ferry N, Lacour B. Serum activity of mitochondrial aspartate aminotransferase: A sensitive marker of alcoholism with or without alcoholic hepatitis. Hepatology. 1984;4:893-896.
Zhou SL, Gordon RE, Bradbury M, Stump D, Kiang CL. Ethanol up-regulates fatty acid uptake and plasma membrane expression and export of mitochondrial aspartate aminotransferase in Hep G 2 cells. Hepatology. 1998;27:1064-1074.
Gazuwa SY, Dabak JD and Ubom GA. Contaminants in local alcoholic beverages: zinc and manganese contamination. Int. J. Biol. Chem. Sci. 2008;2(4):411-416.
Asma S, Kamel K, MedSaleh B. Ethanol exposure induces liver and kidney injury in adult male rabbits. Annals of Biological Research. 2014;5(4):23-26.
Bosma PJ, Chowdhury JR, Bakker C, Gantla S, Boer A, Oostra BA, et al. The genetic basis of the reduced expression of bilirubin UDP glucuronosyltransferase 1 in Gilbert’s syndrome. N Engl J Med. 1995;333:1171.
Selinger MJ, et al. GGT activity in liver disease. Clin Chim. Acta. 1982;125(3):283-287.
Imo C, Uhegbu FO, Ifeanacho NG, Egbeigwe O, Ezekwe AS. Biochemical and histological changes associated with methanolic leaf extract of Gongronema latifolium in acetaminophen-induced hepatic toxicity in wistar albino rats. Int. J. Biomol. Biomed. 2014;4(2):1-7.
Rosalki S. Identifying the alcoholic. In clinical Biochem of Alcoholism, (Ed. Rosalki S) Churchill, Livingstone, Edinburgh. 1984;65-92.
Ireland A, Hartley L, Ryley N. Raised Gamma-Glutamyl-transferase activity and the need for liver biopsy. Br. Med. J. 1991;302:388.
Okoroafor C, Ogbo I, Azi S. The effect of alcohol intoxications on hematological parameters of adult albino wistar rats. International Journal of Community Research. 2013;2(3):46-49.
Esmeralda Thoma, Sonila Bitri, Klodeta Mucaj, Andrin Tahiri, Irida Pano. Changes of Some Blood Count Variables in Correlation with the Time of Alcohol Abuse. J Addict Res Ther. 2015; 6:2.
Moses D. Lugos, Bitrus N. Lekshak, Changjul L. Singnap, Umanka Y. Polit, Benedict H. Mangs. Investigating the impact of alcohol consumption on some haematological variables among alcohol consumers in Jos Metropolis, Nigeria. International Journal of Haematology and Blood Disorder. 2017;3(3):1-6.
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