Changes in Antioxidant Markers and Histopathology of Wistar Rats Fed with Edible Clay (Nzu)

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A. Dokubo
A. E. Okwudike
K. T. Nwauche


The changes in the antioxidant markers and histopathology in both adult male and female wistar rats fed with Calabash Chalk (nzu) was investigated. Twenty (20) wistar rats weighing between (120-150 g) were used for this study. They were randomly divided into four (4) Groups containing five (5) animals each (n=5) A-D. Groups (B-D) were fed with 1.0%, 2.0% and 8.0% of clay. Group A, not fed with clay served as control. After 21 days of continuous feeding, the animals were sacrificed and their liver organs excised for the following antioxidant markers (Catalase CAT, Reduced Glutathione GSH, Superoxide dismutase, SOD) and histopathology. The results showed significant (p< 0.05) increase in catalase activity in rats fed with 1.0% and 8.0% clay compared to control (0 clay) and group fed with 2.0%. However, no significant (p>0.05) difference was obtained for the group fed with 2.0% when compared to control.  The results obtained for GSH and SOD also showed no significant (p>0.05) difference in the fed groups when compared to control. Histopathological changes indicated mild periportal and intraparenchymal inflammation in group fed with 8.0%. From the study it can be deduced that clay consumption has the potential to elicit the activities of antioxidant markers and subsequent depletion providing weak defenses against reactive oxygen species (ROS) and liver damage in the rats.

Calabash chalk, nzu, edible clay, antioxidant markers, activity, histopathology

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How to Cite
Dokubo, A., Okwudike, A. E., & Nwauche, K. T. (2021). Changes in Antioxidant Markers and Histopathology of Wistar Rats Fed with Edible Clay (Nzu). Journal of Applied Life Sciences International, 24(1), 16-20.
Original Research Article


Agomuo EN, Amadi PU, Adumekwe C. Gestational geophagia affects nephrocardiac Integrity, ATP-driven proton pumps, the Renin–Angiotensin–Aldosterone system, and F2- Isoprostane status. Medical Sciences. 2019;7(2):13.

Ikewuchi CC, Ikewuchi JC, Ezeka UK, Ifeanacho MO. Effect of “edible clay” (takere) suspension on serum lipid profiles and atherogenic indices of normal Wistar rats. Food science & nutrition. 2019; 7(3):977-86.

Dean JR, Deary ME, Gbefa BK, Scott WC. Characterisation and analysis of persistent organic pollutants and major, minor and trace elements in Calabash chalk. Chemosphere. 2004;57(1):21-5.

Ekong MB, Akpantah AO, Ibok OS, Eluwa MA, Ekanem TB. Differentia effect of calabash chalk on the histology of the liver of adult Wistar rats. Int J Health. 2009; 8:2.

Ogbuagu DH, Nwachukwu IN, Nwazuluahu AC, Kalakiya NP. Haematotoxicogical and hepatotoxic inductions of a geophagic substance (Calabash chalk) on the albino rat (Rattus norvegicus). Journal of Environmental Toxicology and Public Health. 2017;2:14-26.

Aprioku, JS, Ogwo-Ude EM. Gestational toxicity of Calabash chalk (Nzu) in Wistar rats. International Journal of Applied and Basic Medical Research. 2018;8(4):249-252.

Sofo GA. A Study of the Effects of Clay Eating on Iron Status and Growth Rate Using Rats (Doctoral dissertation, University of Ghana).

The National Population Commission (NPC) 2019.

Sinha AK. Colorimetric assay of catalase. Analytical biochemistry. 1972;47(2):389-94

Ellman GL. Tissue sulphydryl groups. Archives of Biochemistry & Biophysics. 1959; 82:70-77.

Marklund SL and Marklund G. Involvement of superoxide anion radical in the auto- oxidation of pyrogallol and convenient assay for superoxide dismutase. European Journal of Bio-chemistry. 1974;47:469.

He L, He T, Farrar S, Ji L, Liu T, Ma X. Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species. Cellular Physiology and Biochemistry. 2017;44(2):532-53.

Yan S, Wang J, Zhu L, Chen A, Wang J. Toxic effects of nitenpyram on antioxidant enzyme system and DNA in zebrafish (Danio rerio) livers. Ecotoxicology and environmental safety. 2015;122:54-60.