Ameliorative Effects of Dietary Ocimum gratissimum Leafmeal on African Catfish, Clarias gariepinus Subjected to Pathogenic and Transportation-Induced Stress

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Olanipekun O. Samuel
K. Gbadamosi Oluyemi
Adegbenro Muyiwa
A. I. Agbona
S. L. Adebisi

Abstract

The primary aim of this project is the use of functional feed supplements to ameliorate or reduce the effects of stressors rather than using chemicals that could be harmful and expensive.  Specifically, assessment of the ameliorative effect of locally available phytogenic product, Ocimum gratissimum on the negative effects of stress in African catfish production was carried out in a study that lasted for 70 days. Five experimental diets were formulated, at different inclusion levels of O. gratissimum , as 0.00 g (control), 0.05 g, 0.10 g, 0.15 g, 0.20 g per 100 g of diet denoted as T1 (control), T2, T3, T4, and T5 respectively. At the end of the feeding trial, stress-induced treatments of experimental fish were done using pathogenic and transportation stressors. Zootechnical parameters in terms of growth and nutrient utilization were evaluated. Physiological stress assessment (Aspartate transaminase, AST and Alanine transaminase, ALT) and stress biomarkers (cortisol and glucose) were carried out using liver and blood samples from experimental fish. Results showed that zootechnical parameters were significantly (P < 0.05) enhanced with increasing supplementation levels of O. gratissimum. There were better performances in the growth and nutrient utilization indices like weight gain, specific growth rate, protein efficiency and feed conversion ratio as the level of O. gratissimum was increased to 0.15 mg/g in the diets. Curvilinear trends were recorded with a second degree polynomial regression model depicting a strong relationship between SGR and O. gratissimum supplementation in fish diet followed by adverse responses when increased to 0.20 mg/g in the diet. There were significant reduction in stress parameters with increasing supplementation levels of O. gratissimum leaf extract in the diets. The best supplementation level of O. gratissimum was 0.15 mg/g in T4. From the above deductions, this study confirmed the positive ameliorating effects of O. gratissimum on the African catfish during stressful episodes.

Keywords:
Stressors, aquaculture, nutrition, phytogenic, supplementation

Article Details

How to Cite
Samuel, O. O., Oluyemi, K. G., Muyiwa, A., Agbona, A. I., & Adebisi, S. L. (2020). Ameliorative Effects of Dietary Ocimum gratissimum Leafmeal on African Catfish, Clarias gariepinus Subjected to Pathogenic and Transportation-Induced Stress. Journal of Applied Life Sciences International, 23(12), 67-75. https://doi.org/10.9734/jalsi/2020/v23i1230206
Section
Original Research Article

References

Gbadamosi OK, Olanipekun OS. Effects of dietary Goatweed on the nutritional performance profile of African catfish. LRRD. 2020;33:48-54.

FAO. Food and Agricultural Organisation of the United Nations: The state of world fisheries and Aquaculture. Rome, Italy; 2020.

Wedemeyer GA, Barton BA, McLeay DJ. Stress and acclimation. In: Methods for. Fish Biology. (Eds: Schreck, C. and Moyle, P). American Fisheries Society, Bethesda, Maryland. 2000;7:451-489.

Tekle EW, Sahu NP. Growth and immunodulatory response of Nile tilapia, Oreochromis niloticus fingerlings to ethanolic extract of Moringa oleifera flower. IJSRP. 2015;5(7):285-296.

Tejpal CS, Pal AK, Sahu NP, Kumar J, Muthappa NA, Vidya, S Rajan MG. Dietary supplementation of L-tryptophan mitigates crowding stress and augments the growth in Cirrhinus mrigala fingerlings. Aquaculture. 2009;293:272-277.

Li R. Effect of dietary vitamin C on weight gain, tissue ascorbate concentration, stress response, and disease resistance of channel catfish Ictalurus punctatus. J. World Aquaculture Soc. 2009;29(1):1-8.

Chatterjee N, Pal AK, Das T, Manush SM, Sarma K, Venkateshwarlu A, Mukherjee SC. Secondary stress response in Indian major carps Labeo rohita (Ham), Catla catla (Ham) and Cirrhinus mrigala (Ham) fry to increasing packing densities. Aqua. Res. 2006;37:472–476.

Kaleeswarana B, Ilavenilb S. Ravikumara R. Dietary supplementation with Cynodon dactylon (L.) enhances innate immunity and disease resistance of Indian major carp, Catla catla (Ham.). Fish and Shellfish Immunology. 2011;31:953-962.

Sahu S, Das BK, Mishra BK, Pradhan J,Sarangi N. Effect of Magnifera indica kernel as a feed additive on immunity and resistance to Aeromonas hydrophila in Labeo rohita fingerlings. Fish Shellfish Immunol. 2001;23:109-118.

Soosean C, Marimuthu K, Sudhakaran S, Xavier R. Effect of Mangosteen (Garcinia mangostana L.) extracts as a feed additive on growth and haematological parameters of African catfish (Clarias gariepinus) fingerlings. European Review of Medicine and Pharmaceutical Science. 2001;14: 605-611.

Dada AA, Sonibare OF. Effect of dietary administration of the herbal additive siamweed, Chromolaena odorata on growth performance and haematological changes in Clarias gariepinus fingerlings. Journal of Fisheries International. 2015; 3(1):221-226.

Sofowora A. Botanical herbarium of medicinal plants in Africa. John Wiley and Sons, New York. 2014;242-246.

Gbadamosi OK, Salako FO. Gustation and growth performance of African Catfish, Clarias gariepinus fed varying levels of dietary African Basil, Ocimum gratissimum leaf supplementation. Egyptian Academic Journal of Bioscience. 2016;6:9-15.

AOAC. Association of official analytical chemists. Official methods of analysis. (18th ed.). Arlington, VA, USA; 1990.

Hardy RW, Sullivan CV. Pathogenesis of infectious diseases of fish. Ann. Rev. Microbiol. 2003;40:479-502.

Sudagar M, Hajibeglou A. Effect of plant extracts supplemented diets on immunity and resistance to Aeromonas hydrophila in common carp (Cyprinus crpio). Research Journal of Animal Sciences. 2010;4(1):26-34.

Adebayo OT. Reproductive performance of African clariid catfish, Clarias gariepinus broodstocks on varying maternal stress. J. Fish. Int. 2006;1:17-20.