Bacteria Screening with Sufficient Nitrate Extraction from Wastewater

Main Article Content

A. Y. Bello
A. Nafi’u
Z. A. Haruna
A. H. Ridwan
A. Mohammed
M. T. Ibrahim

Abstract

Search for effective biological strain for use to remedy water pollution has been limited. This study was performed to search for effective bacteria capable of extracting nitrate from wastewater. Samples from abattoir inside (Abi) and abattoir outside (Abt), fishpond of kwalkwalawa (Fpk) and mabera fishpond (Fpm) wastewater were used. Using conventional standard plate technique as 1.96×107 cfu/ml (Abi) had highest bacteria count while 1.05×107 cfu/ml (Fpk) had minimal. The obtained pure colonies were morphologically and biochemically characterized including two Enterobacter specie, Proteus and Pseudomonas specie. The isolated organisms were used as a test organism for the removal of nitrate present in high nitrate containing medium. Prior to the extraction, the wastewater samples were physicochemically analyzed using parameters like color, odor, pH, alkalinity, hardness, temperature and nitrate. For nitrate extraction, bacteria were inoculated in a separate tubes containing nitrate broth, incubated in a rotary shaker 150rpm at 37°C for 16hrs. The supernatant from 10minutes centrifuged culture were used for nitrate removal evaluation after a series of reaction and stop using spectrophotometer at 420nm. The absorbance 0.732 proteus specie after estimation of nitrate in a medium was maximal while Enterobacter specie 0.574 was minimal. Meanwhile, two of the Enterobacter specie has similar absorbance 0.012 while Proteus and Pseudomonas species had 0.016 and 0.010 respectively in the nitrate concentration removed within the media. The nitrate present in the medium was compared with a known standard curve prepared with NaNO3 at 100-1000 ppm. The result indicated that bacteria from fish pond wastewaters had highest potential for extracting nitrate and that inherent bacteria are capable of removing pollutant nitrate and the bacteria may be able to remove nitrate efficiently if genetically engineered.  

Keywords:
Nitrate, wastewater, removal, spectrophotometer and remediation

Article Details

How to Cite
Bello, A. Y., Nafi’u, A., Haruna, Z. A., Ridwan, A. H., Mohammed, A., & Ibrahim, M. T. (2020). Bacteria Screening with Sufficient Nitrate Extraction from Wastewater. Journal of Applied Life Sciences International, 23(12), 52-58. https://doi.org/10.9734/jalsi/2020/v23i1230204
Section
Original Research Article

References

Sahset T, Nuhi D, Yalcin S. The effect of pH on phosphate removal from wastewater by electro-coagulation with iron plate electrode. J. Hazard. Mater. 2006;137: 1231-1235.

Amir HM, Ali RM, Farham K. Nitrogen removal from wastewater in a continuous flow sequencing batch reactor. Pakistan J. Biol. Sci. 2004;7(11):1880-1883.

Jacqueline DE, Barry CL. Regeneration of phosphorus and nitrogen by 4 species of heterotrophic nanoflagellates feeding on 3 nutritional status of a single bacterial strain. Appl. Environ. Microbiol. 1995. 16(3):1033-1038.

Krishnaswamy U, Muthusamy M, Perumalsamy L. Studies on the efficiency of the removal of phosphate using bacterial consortium for the biotreatment of phosphate wastewater. Eur. J. Appl. Sci. 2009;1:6-15.

Nakasone H, Yamamoto T. The impacts of the water quality of the inflow water from tea field son irrigation reservoir ecosystems. Pad. Water Enviro. 2004;2: 45-50.

Filintas A, Dioudis P, Stamatis G, Hatzopoulos J, Karyotis T. Environmental assessment of Groundwater nitrate pollution from agricultural wastes and fertilizers in central Greece watersheds using remote sensing and GIS. Proceedings of the 3rd International Conference AQUA 2008 on: Water Science and Technology with Emphasis on Water and Climate, Oct. 16-19, Athens, Greece. 2008;1-10.

Jin Z, Chen Y, Wang F, Ogura N. Detection of nitrate sources in urban ground water by isotopic and chemical indicators, Hangzhou City, China. Enviro. Geo. 2004;45:1017-1024.

Wakida FT, Lerner DN. Potential nitrate leaching to groundwater from house building. Hydro. Process. 2006;20:2077-2081.

Singleton MJ, Woods KN, Conrad ME, DePaolo DJ, Dresel PE. Tracking sources of unsaturated zone and groundwater nitrate contamination using nitrogen and oxygen stable isotopes at the Hanford site. Washington J. Enviro. Sci. Tech. 2005;10:3563-3570.

Eckford RE, Fedorak PM. Planktonic nitrate-reducing bacteria and sulfate-reducing bacteria in some western Canadian oil field waters. J. Ind. Micro. Biotech. 2002;29:83-92.

Weber KA, Picardal FW, Roden EE. Microbially catalyzed nitrate dependent oxidation of biogenic solid phase Fe (II) compounds. Enviro. Sci. Tech. 2001; 35:1644-50.

Zahoor A, Rehman A. Isolation of Cr (VI) reducing bacteria from industrial effluents and their potential use in bioremediation of chromium containing wastewater. J. Enviro. Sci. 2009;21(6):814-820.

Neha S, Aditi D. Bioremediation of dairy wastewater for nitrate reduction. Wor. J. Pharm. L. Sci. 2017;3(1):375-384.

Bello AY, Mohammed SSD, Ijah UJJ. Screening and molecular identification of fungi isolated from soil with potential for bioremediation of tannery waste polluted soil. Equ. J. Sci. Tech. 2020; 7(1):06 – 15.

Oyeleke SB, Manga SB. Essential of laboratory practicals in microbiology. First edition, Tobest Publishers, Minna Nigeria. 2008;36-67.

Oguferu AG, Ologbosere OA, Aluyo HAS, Beshiru A, Otneje FI. Physiochemical and microbiology profile of bacterial and fungal isolate of Ikpoba River in Benin City: Public Health implication. Afr. J. Enviro. Sci. Tech. 2016;10 (3):67-76.

Ogbomida ET, Kubeyinje B, Ezemonye LI. Evaluation of bacterial profile and biodegradation potential of abattoir wastewater. Afr. J. Enviro. Sci. Tech. 2016; 10(2):50-57.

Ire FS, Amos MO, Ossia-Ndidi LN. Microbiological and physiochemical assessment of abattoir effluents and receiving water bodies in Port Harcourt. J. Pharm. Clin. Bio. Sci. 2017;5(1):34-39.

Drysdale GD, Kasan HC, Bux F. Denitrification by heterotrophic bacteria during activated sludge treatment. Water SA. 1999;25(3):357-362.

Shirey JJ, Sexstone AJ. Denitrification and nitrate-reducing bacterial populations in abandoned and reclaimed minesoils. FEMS Micro. Eco. 1989;5(1):59- 69.

Lalucat J, Bennasar A, Bosch R, García-Valdés E, Palleroni NJ. Biology of Pseudomonas stutzeri. Micro. Mol. Bio. Rev. 2006;70(2):510-547.

Rezaee A, Godini H, Dehestani S, Kaviani S. Isolation and characterization of a novel denitrifying bacterium with high nitrate Removal. J. Enviro. H. Sci., England. 2010;7(4):313-318.