Response of Some Cameroonian Cocoyam Xanthosoma sagittifolium (L.) Schott. Cultivars/ Landraces to Tissue Culture Techniques

Main Article Content

Dominic Kumbah Njualem
Tange Denis Achiri
Tiozang Nangni Florente
Abdulai Assan Nkuh
Eugene Lendzemo Tatah
Fornkwa Victorine Yaya

Abstract

Aims: This study was aimed at exploring tissue culture technique as a tool for mass propagation of some Cameroonian cocoyam (Xanthosoma sagittifolium) cultivars/landraces (red, yellow and white skin colour).

Study Design: The experiment was laid out in a completely randomized design with three treatments in four replications.

Place and Duration of Study: The study was conducted in the tissue culture laboratory of the Institute of Agricultural Research for Development, Bambui, Cameroon, in the first half of                  2018.

Methodology: Explants were gotten from three Cameroonian cocoyam landraces (red, white and yellow skin colour). Shoot tips were excised and cultured on Murashige and Skoog (MS)                  medium supplemented with 30 g of sucrose, 5ml of ascorbic acid, 4ml of 6- benzylaminopurine (BAP 1mg/l), 1 ml indole-3- acetic acid (IAA 1 mg/l) and 6 g of agar at pH of 5.8±0.1 for shoot initiation and proliferation. Data was collected after 4 weeks (number dead, number rooted, number of roots, number of buds) and 12 weeks (number of leaves, shoot length, number contaminated) of initiation.

Results: All the landraces responded positively to the growth media since none died. The number of explant rooted did not vary significantly (p > .05). The highest number of roots and buds were from the white cultivar, followed by the red cultivar. Analysis of variance revealed significant differences (p = .05) in most of the parameters measured except for number rooted. Highest numbers of leaves and shoot length were recorded from the red cultivar, followed by the yellow and white cultivars. However, the white cultivar (4.2) was more susceptible to pathogen than the yellow (3.5) and red (2.67) cultivar (F = 19.13, df = 2, 8, p < .001.

Conclusion: Cameroonian cocoyam cultivars responded positively as far as growth parameters are concern on growth media. It is recommended that the three cocoyam cultivars be followed from growth media to the field and evaluate their growth and yield parameters.

Keywords:
Cocoyam, cultivar, explant, tissue culture, IRAD Bambui, Xanthosoma sagittifolium.

Article Details

How to Cite
Njualem, D. K., Achiri, T. D., Florente, T. N., Nkuh, A. A., Tatah, E. L., & Yaya, F. V. (2020). Response of Some Cameroonian Cocoyam Xanthosoma sagittifolium (L.) Schott. Cultivars/ Landraces to Tissue Culture Techniques. Journal of Applied Life Sciences International, 23(9), 1-9. https://doi.org/10.9734/jalsi/2020/v23i930182
Section
Original Research Article

References

Damilola O, Bodunde OJ, Olufemi AA, Amoo IA. Chemical composition of red and white cocoyam (Colocosia esculenta) leaves. Int. J. Sci and Res. 2013;2(11):121-126.

Alamu S, McDavid CR. Production of flowering in edible aroids by gibberellic acid. Trop. Agric. 1978;55:81-86.

Pinto FJ, Onwaeme JB. Food values of breadfruit, taro leaves, coconut and sugar cane”. FAO plant production and protection paper 126, FAO. Rome. 2000;268.

Onwueme IC, Charles WB. Utilization, socio-economic perspective and future prospects. In: Onwueme IC, Charles WB, editors. Tropical root and tuber crops: production, perspectives and future prospects. FAO Plant Production and Protection Paper. 126th ed. Rome: FAO; 1994.

Owusu-Darko PG, Paterson A, Omenyo EL. Cocoyam (corms and cormels) an underexploited food and feed resource. J Agric Chem Environ. 2014;03(01):22-29.

Grid MC. Minerals: Dietary need absorption, transport and excretion - Workshop Seminar. 2006;21-24.

Lewu MN, Adebola PO. Comparative assessment of the nutritional value of commercially available cocoyam and potato in South Africa. J Food Qual. 2010;33:461-476.

Gudjonsdottir M, Boakye AA, Wireko-Manu FD, Oduru I. Characterization of red and white cocoyam (Xanthosoma sagittifolium) roots, flours and starches during heating by low field NMR. Proceedings of the XIII International Conference on the Application of Magnetic Resonance in Food Science. 2016;49-53. DOI: 10.1255/mrfs.10

Wesphal E, Embrecats J, Ferweerda JD, Van Gils-Meeus HAE, Mutsaers HJW, Wesphal SJMC. Cultures tropicales avec reference special au Cameroon. Pudoc Wageningen, Hollande; 1985.

Adelekan BA. An evaluation of the global potential of cocoyam (Colocasia and Xanthosoma species) as an energy crop. Br J Appl Sci Technol. 2012;2(1):1-15.

Perez EE, Breene WM, Bahnassey YA. Gelatinization profiles of Peruvian carrot, cocoyam and potato starches as measured with the Brabender viscoamylograph, rapid visco-analyzer, and differential scanning calorimeter. Starch-Stärke. 1998;50(1): 14–16.

Sefa-Dedeh S, Kofi-Agyir Sackey E. Starch structure and some properties of cocoyam (Xanthosoma sagittifolium and Colocasia esculenta) starch and raphides. Food Chemistry. 2002;79(4):435–444. Available:http://doi.org/10.1016/S0308-8146(02)00194-2

Sefa-Dedeh S, Agyir-Sackey EK. Chemical composition and the effect of processing on oxalate content of cocoyam Xanthosoma sagittifolium and Colocasia esculenta cormels. Food Chemistry. 2004;85(4):479–487. Available:http://doi.org/10.1016/S0308-8146(02)00244-3

Ndabikunze BK, Talwana HAL, Mongi RJ, Issa-Zacharia A, Serem AK, Palapala V, Nandi JOM. Proximate and mineral composition of cocoyam (Colocasia esculenta L. and Xanthosoma sagittifolium L.) grown along the Lake Victoria basin in Tanzania and Uganda. Afr. J. Food. Sci. 2011;5(4):248–254.

Falade KO, Okafor CA. Physical, functional, and pasting properties of flours from corms of two cocoyam (Colocasia esculenta and Xanthosoma sagittifolium) cultivars. J Food Sci Technol. 2014;52(6): 3440-3448. Available:http://doi.org/10.1007/s13197-014-1368-9

Mbouobda HD, Boudjeko T, Djocgoue PF, Tsafack TJJ, Omokolo DN. Morphological characterization and agronomic evaluation of cocoyam (Xanthosoma sagittifolium L. Schott) germplasm in Cameroon. J Biol Sci. 2007;7(1):27-33.

Schafer LL. Improvement of cocoyam (Xanthosoma sagittifolium L. Schott) growing system in Bamilike land (West-Cameroon). Cahier Agriculture. 1999;8:9-20.

Xu T, Omokolo ND, Tsala NG, Ngonkeu MEL. Identification of the causal agent of cocoyam root rot disease in Cameroon. Acta Mycol. Sin. 1995;14:37-45.

Nzietchueng S. Xanthosoma sagittifolium (cocoyam) genus and production constraints: Case of root rot cause by Pythium myriotylum Drechsl in Cameroon. PhD Thesis. University of Yaounde, Cameroon; 1985.

Ngouo LV, Nzietchueng S, Valet G. Inflorescence and floral organization, fertility and pollen germination at three Xanthosoma sp. cultivated in Cameroon. Agro. Afri. 1989;1:95-104.

Njualem DK, Achiri TD, Yuninwenkeh AC, Abdulai AN, Yaya FV. Enhancing micropropagation of some Cameroonian plantain (Musa paradisiaca L) cultivars/landraces. Int J Plant and Soil Sci. 2019;28(6):1-10.

Toledo J, Nelson Espinoza, Ali Golmirzaie. Management of in vitro Plantlets in potato seed production. Training manual. International Potato Center; 1998.

Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 1962;15:473-497.

Onokpise OU, Wutoh JG, Ndzana X, Tambong JT, Meboka MM. Evaluation of cocoyam germplasm in Cameroon. In: Jamick, J., Perspective of new crops and new uses. Edited by ASHS Press. Alexandra, VA. 1999;394-396.

Oguntowo O, Obadina AO, Sobukola OP, Adegunwa MO. Effects of processing and storage conditions of cocoyam strips on the quality of fries. Food Science and Nutrition. 2016;4(6):906-914.

Prakash S, Savangikar VA. Low cost option for tissue culture technology in developing countries. Proceedings of Technical Meeting, August 26-30, FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna. 2002:32-45.

Vuylsteke D, De Langhe E. Feasibility of in vitro propagation of bananas and plantains. Tropical Agriculturist. (Trinidad). 1985;62:323-328.

Rahman S, Biswas N, Hassan MM, Ahmed AG, Mamun ANK, Islam MR, Moniruzzaman M, Haque ME. Micropropagation of banana (Musa sp.) cv. Agnishwar by in vitro shoot tip culture. Intl. Res. J. Biotech. 2013;4(4):83-88.

Ferdous MH, Masum BAA, Mehraj H, Taufique T, Jamal-Uddin AFM. BAP and IBA pulsing for in vitro multiplication of banana cultivars through shoot-tip culture. J Biosci Agric Res. 2015;03(02):87-95.

Al-Sadi AM. Variation in resistance to spot blotch and the aggressiveness of Bipolaris sorokiniana on barley and wheat cultivars. J Plant Pathol. 2016;98:97-103. DOI: 10.4454/jpp.v98i1.029