Isolation and Identification of Antibiotic Producing Bacteria from Soil Samples of Abattoir in Lapai, Niger State, Nigeria

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E. I. Uwalaka
Z. J. Kassim
A. R. Abdulmumin
A. Hamzat


Antimicrobial agents or antibiotics are the most significant commercially available and utilized secondary metabolites, which are highly produced by the soil microbes (bacteria and fungi) and found to be effective and broad spectrum. Microbes produce metabolic products (antimicrobial agents) through the process called antibiosis. Majority of the classes of antibiotics used are derivatives of animals (microbes) and floras (plants). But currently, the microbial resistance is at the top gear which requires more effort to come up with novel structure, effectual, toxic free and reasonable cost of new antimicrobial products against microbial infections. In the present study, a trial was made to isolate, identify and characterize the antibiotic producing bacteria from the soil samples collected from different sites of abattoir in Lapai, using the standard microbiological techniques. A total of nine (9) bacterial of both groups (Gram positive and negative) which includes Corynebacterium diphtheriae, Bacillus cereus, Escherichia coli, Enterobacter aerogene, Clostridium specie, Bacillus subtilis, Pseudomonas species, Staphylococcus aureus and Klebsiella pneumoniae were isolated in the course of the study. Pseudomonas species was the most frequently isolated bacteria (33.33%) while the rest of the isolates were 8.33% across the five different sampling sites. These isolates were further screened against some pathogenic microbes viz, Salmonella sp, Klebsiella pneumoniae, Pseudomonas aeruginosa and Candida albicans. Three of the bacterial isolates isolated from the abattoir were found to exhibits antimicrobial activity against two pathogenic bacteria used in this study. This study indicates that some of the soil microorganisms could be an interesting source of antimicrobial bioactive substances.

Bacteria, antibiotics, soil, antimicrobials, biosynthesizing, inhibition.

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Uwalaka, E. I., Kassim, Z. J., Abdulmumin, A. R., & Hamzat, A. (2019). Isolation and Identification of Antibiotic Producing Bacteria from Soil Samples of Abattoir in Lapai, Niger State, Nigeria. Journal of Applied Life Sciences International, 22(2), 1-8.
Original Research Article


Chaudhary HS, Yadav J, Shrivastava AR, Singh S, Singh AK, Gopalan N. Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India). Journal of Advanced Pharmaceutical Technology Research. 2013;4(2):118-123.

Ruchika S, Swarnjeet K. Antimicrobial and phytochemical screening of trikutatraditional food of western Rajasthan. Indian Journal of Traditional Knowledge. 2017;16:270-276.

Abdulmumin AR, Kassim ZJ, Mohammed SB, Yakubu Y, Ibrahim K. In-vitro Antimicrobial Susceptibility and Phytochemical Constituents of Methanol Leaf Extract of Prosopis africana against Some Selected Microorganisms. Journal of Advances in Microbiology. 2019;18(1):1-8.

Walsh C. Antibiotics: Actions, origins, resistance. American Society for Microbiology (ASM); 2011.

Abdulmumin AR, Kassim ZJ, Muhammad IL, Mohammed SB and Ya’aba Y. Antimicrobial Potency and Bioactive Ingredients of Different Extracts of Prosopis africana against Some Selected Human Pathogenic Microbes: An In-vitro Approach. Journal of Complementary and Alternative Medical Research. 2019;8(1): 1-9.

Nikodinovic J, Barrow KD, Chuck JA. High frequency transformation of the amphotericin- producing bacterium Streptomyces nodosus. Journal of Microbiological Methods. 2003;55(1):273-277.

Basavaraj K, Nanjwade S, Chandrashe K, Prakash SG, Ali MS, Fakirappa VM. Production of Antibiotics from Soil-Isolated Actinomycetes and Evaluation of their Antimicrobial Activities. Tropical Journal of Pharmaceutical Research. 2010;9(4):373-377.

Baltz RH. Marcel Faber Roundtable, is our antibiotic pipeline unproductive because of starvation, constipation or lack of inspiration? Journal of Industrial Microbiology and Biotechnology. 2006; 33(7):507-513.

Smith JE. Perspective in biotechnology and applied microbiology, Murray Moo-Young. 1989;105-134.

Bulock J. Basic Biotechnology. Academic Press. 1987;425-448.

Vandemme EJ. Biotechnology of Industrial Antibiotics. Dekker Series, Vol. 22, Marcel Dekker Inc., New York. 1948;3-42.

Singh AP, Mishra S. Isolation and biochemical characterization of antibiotic producing microorganism from waste soil samples of certain industrial areas of India. Scientific Research Journal of Pharmaceutical Biological Science. 2013; 5(6):80-9.

Makut M, Owolewa O. Antibiotic-producing fungi present in the soil environment of Keffi metropolis, Nasarawa state, Nigeria. Eubacteria. 2011;10(18):19.

Griffith RS. Introduction to vancomycin. Rev. Infect. Dis. 3, 200–204. Doi:10.1093/clinids/3.Supplement_2.S200

Umezawa H, Ueda M, Maeda K, Yagishita K, Kondo S, Okami Y, et al. Production and isolation of a new antibiotic: Kanamycin. Journal of Antibiotics. 1957; 10(1):1-8.

Staunton J, Wilkinson B. Biosynthesis of erythromycin and rapamycin. Chem. Rev. 1997;97:2611–2630.

Tiwari K, Gupta RK. Diversity and isolation of rare actinomycetes: an overview. Critical Revision of Microbiology. 2013;39:256–294.

Sumi CD, Yang BW, Yeo IC and Hahm YT. Antimicrobial peptides of the genus Bacillus: a new era for antibiotics. Can. Journal of Microbiology. 2015;61:93–103.

Mukherjee K, Mandal S, Mukhopadhyay B, Mandal NC, Sil AK. Bioactive compound from Pseudomonas synxantha inhibits the growth of Mycobacteria. Microbiology Research. 2014;169:794–802.

de Oliveira AG, Spago FR, Simionato AS, Navarro MOP, da Silva CS, Barazetti AR, et al. Bioactive organocopper compound from Pseudomonas aeruginosa inhibits the growth of Xanthomonas citri sub sp. citri. Front. Microbiol. 2016;7:113.

Kaur S, Kaur J, Pankaj PP. Isolation and characterization of antibiotic producing microorganisms from soil samples of a certain area of Punjab region of India. International Journal of Pharmaceutical and Clinical Research. 2014;6(4):312-521.

Kavitha R, DhAmodharan N, Dhivya C. Screening, Isolation and Antibacterial Activity of Antibiotic Producing Bacteria Obtained from Saprophytic Soil Samples. Asian Journal of Pharmaceutical and Clinical Research. 2017;10(3):92-96.

Czaplewski L, Bax R, Clokie M, Dawson M, Fairhead H, Fischetti VA, Foster S, Gilmore BF, Hancock RE, Harper D, Henderson IR, Hilpert K, Jones BV, Kadioglu A, Knowles D, Olafsdottir S, Payne D, Projan S, Shaunak S, Silverman J, Thomas CM, Trust TJ, Warn P, Rex JH. Alternatives to antibiotics-a pipeline portfolio review. Lancet Infect Dis. 2016; 16(2):239-251.

Ruan J. Bergey's Manual of Systematic Bacteriology (second edition) Volume 5 and the study of Actinomycetes systematic in China. 2013;53(6):521-530.

Musliu A, Salawudeen W. Screening and Isolation of the soil Bacteria for Ability to produce Antibiotics. 2012;4(5):211-215.

Begum K, Mannan SJ, Rahman MM, Mitchell AA, Opoku R, et al. Identification of Antibiotic Producing Bacteria from Soil Samples of Dhaka, Bangladesh. Journal of Microbiology and Experiment. 2017);4(6): 1-5.

Rafiq A, Khan SA, Akbar A, Shafi M, Ali I, Rehman FU, Rashid R, Shakoor G, Anwar M. Isolation and identification of antibiotic producing microorganisms from soil. International Journal of Pharmaceutical Sciences & Research. 2018;9(3):1002-1110.

Hassan AS, Erum H, Raheela RZ. Isolation and screening of soil bacteria for potential antimicrobial activity. Federal Urdu University of Arts, Science and Technology Journal of Biology. 2014;4(2):217-219.

Samuel L, Chai SL, Ismail A, Fazia MS, Ng Kok H, Awang A. Antimicrobial producing microbes isolated from soil samples collected from Nanga Merit Forest in Sarawak, Malaysian Borneo. European Journal of Experimental Biology. 2014; 4(1):494-501.

Rajnish V. Isolation, Identification, Purification, and Characterization of Antibiotic-Producing Bacteria from Different Soil Samples. International Journal of Advance Research, Ideas and Innovations in Technology. 2017;3(5):196-204.

Sharga BM, Nikolaychuk VI, Maga MI. Comparative IR Spectrometry of antimicrobial substances derived antibiotic from Bacillus. Mikpobiojiotir. 2004;15:75-77.

Hassan SA, Hanif E, Zohra RR. Isolation and screening of soil bacteria for potential antimicrobial activity. Fuuast Journal of Biology. 2014;4(2):217-219.

Sethi S, Ravi K, Saksham G. Antibiotic Production by Microbes Isolated from Soil. Journal of Applied Pharmaceutical Sciences. 2013;4(5):2967-2973.