The Climbing Performance, Neuromuscular Transmitter (ACHE) Activity, Reproductive Performance and Survival of Drosophila melanogaster Fed Diet with Mangifera indica Cold Aqueous Leaf Extract

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Etuh Monday Alexander
John Chinyere Aguiyi
Iorjiim Walter Mdekera
Ochala Sunshine Ogwu
Oyeniran Oluwatosin Imoleayo
Chinelo Vera Ugokwe
Dung Pam


Objective: To screen the toxic effect of Mangifera indica aqueous leaf extract in Drosophila melanogaster.

Materials and Methods: Phytochemical screening was carried out. 20 Adult flies were exposed to 7.5 mg, 15 mg,  30 mg, 45 mg and 100 mg /10 g diet for acute toxicity (168hrs) while 50 flies were exposed to 2.5 mg, 5 mg and 10 mg/10 g diet for sub chronic (28 days). All concentrations were prepared in 1000µl of distilled water and replicated three (3) times. Diet+1000µl of Distilled water served as control. Fecundity/developmental toxicity, Climbing and AChE activities were carried out by exposing flies to the sub-chronic concentrations for 5 days.

Results: Phytochemical screening revealed the presence of alkaloids, phenols, flavonoids, tannins, saponins and terpenoids. The acute toxicity test showed 100% mortality at 100 mg/ 10 g diet and 168hrs LC50 was 72.4 mg/10 g diet. The sub chronic toxicity test showed decrease in flies survival along concentration with a least survival at 10 mg/10 g diet. There was a slight reduction and elevation in the Climbing and AChE activities respectively but not statistically significant (p>0.05) compared to control. At 5 mg and 10 mg/10 g diet there was a delay in the development with few emerged flies.

Conclusion: From the Results, it can be concluded that Mangiferaindica aqueous leaf extract may be toxic at high dose from 72.4 mg/10 g diet and might have an adverse effect on the development and survival of flies at sub chronic concentration as low as 2.5 mg/10g diet.

Neuromuscular transmitter (ACHE) activity, reproductive performance, Drosophila melanogaster, Mangifera indica, aqueous leaf extract.

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Alexander, E. M., Aguiyi, J. C., Mdekera, I. W., Ogwu, O. S., Imoleayo, O. O., Ugokwe, C. V., & Pam, D. (2019). The Climbing Performance, Neuromuscular Transmitter (ACHE) Activity, Reproductive Performance and Survival of Drosophila melanogaster Fed Diet with Mangifera indica Cold Aqueous Leaf Extract. Journal of Applied Life Sciences International, 22(2), 1-11.
Original Research Article


Diallo D, Paulsen BS, Liljebäck TH, Michaelsen TE. The malian medicinal plant Trichiliaemetica, studies on polysaccharides with complement fixing ability. Journal of Ethnopharmacology. 2003;84:279–287

Gurib-Fakim A. Medicinal plants: Traditions of yesterday and drugs of tomorrow. Molecular aspects of Medicine. 2006;27(1):1-93.

Walker A, Sillans R. Les plantesutiles du Gabon. Paul Lechevalier, Paris; 1961.

Sandhya B, Thomas S, Isabel W, Shenbagarathai R. Ethnomedicinal plants used by the Valaiyan community of Piranmalai hills (Reserved forest), Tamilnadu, India. A pilot study. Complementary and Alternative Medicines. 2006;3:101-114

Linderman JA, Chambers MC, Gupta AS, Schneider DS. Infection-related declines in chill coma recovery and negative geotaxis in Drosophila melanogaster. PLoS One. 2012;7(9):41907.

Rosenthal L. Medicinal monday- Herbs to avoid while trying to conceive. Path to fertility; 2014.


Kelly JG. (n’d). How Medications Can Affect your Fertility; 2018.

Frohne P. A color atlas of poisonous plants. a hand book for pharmacists, doctors, toxicologists and biologists. A Wolfe Science Book, Wolfe Publishing LHd London W1 P6HE; 1984.

[ISBN 07234 0839:4]

Akachukwu D, Okafor PN, Ibegbulem CO. Phytochemical content of Cnidoscolus aconitifolius leaves and toxicological effect of its aqueous leaf extract in Wistar rats. American Journal of Physiology, Biochemistry and Pharmacology. 2014;3 (1):26 31.

Celik TA. Potential genotoxic and cytotoxic effects of plant extracts. In A compendium of essays on alternative therapy. Intech Open; 2012.

Ishii R, Yoshikawa K, Minakata H, Komura H, Kada T. Specificities of bio-antimutagens in plant kingdom. Agricultural and biological chemistry. 1984;48(10): 2587-2591.

Etuh MA, Aguiyi JC, Ochala SO, Simeon O, Oyeniran OI, Debola OO, Pam D. The In vivo antioxidant protective activity of Mangifera indica cold aqueous leaf extract in Drosophila melanogaster. Journal of Advances in Biology & Biotechnology. 2019;1-7.

Dweck AC. Article for cosmetics and toiletries magazine ethnobotanical plants from Africa. Black Medicare Ltd, Iltshire, UK; 2001.


Wauters G, Charlier J, Janssens M. Agglutination of pYV+ Yersinia enterocolitica strains by agglutinin from Mangiferaindica. Journal of clinical microbiology. 1995;33(3):772-774.

Hirte M. Benefit of Mango for the Human Health; 2002.


Somkuwar DO, Kamble VA. Phytochemical screening of ethanolic extracts of stem, leaves, flower and seed kernel of Mangifera indica L. Int. J. Pharm. Bio. Sci. 2013;4(2):383-389.

Amy R, David F, Elizabeth M, Kailash G, Katherine S, GregoryC, Michael G. William M, Deborah M. Alternative methods for the median lethal dose (LD50) test: The up-and-down procedure for acute oral toxicity. ILAR Journal. 2002; 43:233–243.

Giacomotto J, Ségalat L. High‐throughput screening and small animal models, where are we? British Journal of Pharmacology. 2010;160(2):204-216.

Festing MFW, Baumans V, Combes DR, Halder M, Hendriksen FM, Howard BR, Lovell DP, MooreGJ, Overend P, Wilson MS. Reducing the use of laboratory animals in biomedical research: Problems and possible solutions. Alternative Laboratory Animal. 1999;26:283–301.

Chien S, Reiter LT, Bier E, Gribskov M. Homophila: Human disease gene cognates in Drosophila melanogaster. Nucleic acids research. 2002;30(1):149-151.

Chintapalli VR, Wang J, Dow JA. Using FlyAtlas to identify better Drosophila melanogaster models of human disease. Nature Genetics. 2007;39(6):715.

Pawson T, Bernstein A. Receptor tyrosine kinases: Genetic evidence for their role in Drosophila and mouse development. Trends in Genetics. 1990;6:350-356.

Kandel E, Abel T. Neuropeptides, adenylyl cyclase and memory storage. Science-New York then Washington. 1995;825-825.

Whitworth AJ, Wes PD, PallanckLJ. Drosophila models pioneer a new approach to drug discovery for Parkinson's disease. Drug Discovery Today. 2006;11 (3-4):119-126.

Ségalat L. Invertebrate animal models of diseases as screening tools in drug discovery. ACS Chemical Biology. 2007; 2(4):231-236.

Golombieski RM, Graichen DÂS, Rocha JBTD, ValenteVLDS, Loreto ÉLDS. Over-activation of the Drosophila melanogaster hsp83 gene by selenium intoxication. Genetics and Molecular Biology. 2008;31 (1):128-135.

Podder S, Roy S. Exposure-dependent variation in cryolite induced lethality in the non-target insect, Drosophila melanogaster. Interdisciplinary Toxicology. 2014;7(1):17-22.

Wangchuk P, Keller PA, PyneSG, Taweechotipatr M, Tonsomboon A, Rattanajak R, Kamchonwongpaisan S. Evaluation of an ethnopharmacologically selected Bhutanese medicinal plants for their major classes of phytochemicals and biological activities. Journal of Ethnopharmacology. 2011;137(1):730-742.

Abolaji AO, Kamdem JP, Lugokenski TH, Nascimento TK, Waczuk EP, FarombiEO, Rocha JBT. Involvement of oxidative stress in 4-vinylcyclohexene-induced toxicity in Drosophila melanogaster. Free Radical Biology and Medicine. 2014;71:99-108.

Rauser CL, Tierney JJ, Gunion SM, Covarrubias GM, Mueller LD, Rose MR. Evolution of late‐life fecundity in Drosophila melanogaster. Journal of Evolutionary Biology. 2006;19(1):289-301.

Ellman GL, Courtney KD, Andres Jr V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology. 1961;7(2):88-95.

Stalikas CD. Extraction, separation, and detection methods for phenolic acids and flavonoids. Journal of Separation Science. 2007;30(18):3268-3295.

Kumar S, Yadav A, Yadav M, Yadav JP. Effect of climate change on phytochemical diversity, total phenolic content and in vitro antioxidant activity of Aloevera (L.) Burm. f. BMC Research Notes. 2017;10(1):60.

Vishnu R, Nisha R, Jamuna S, Paulsamy S. Quantification of total phenolics and flavonoids and evaluation of in vitro antioxidant properties of methanolic leaf extract of Tarennaasiatica - An endemic medicinal plant species of Maruthamali hills, Western Ghats, Tami Nadu. Journal Research of Plant Science. 2013;2(2):196-204.

Ciepielewska D, Kordan B, Nietupski M. Effect of plant extracts on some stored-product insect pests. Polish Journal of Natural Sciences. 2005;18:7-14.

Roy B, Amin MR, Uddin MN, Islam ATMS, Islam MJ, Halder BC. Leaf extracts of shiyalmutra (Blumea lacera) against lesser grain borer and rice weevil. Journal of Biological Sciences. 2005;5:201-204.

Roy B, Jalal S, Sarker BC, AminMR, JeonYH, Chun IJ. Evaluation of insecticidal activities of common cocklebur fruit extract against pulse beetle Callosobruchus chinensis (Coleoptera: Bruchidae). Korean Journal of Applied Entomology. 2012;51: 325-329.

Roy B, Sarker BC, AminMR, Roy BC, Jalal S. Bioefficacy of shiyalmutra leaf extract again rice weevil. Journal of Science and Technology. 2010;8:1-5.

Ahad MA, Nahar MK, AminMR, SuhSJ, Kwon YJ. Biological activities of five weed extracts against Callosobruchus chinensis L. (Coleoptera: Bruchidae). Korean Journal of Applied Entomology. 2015;54:83-89.

Ahad MA, Nahar MK, AminMR, SuhSJ, Kwon YJ. Effect of weed extracts against pulse beetle, Callosobruchus chinensis L. (Coleoptera: Bruchidae) of mung bean. Bangladesh Journal of Agricultural Research. 2016;41:75-84.

Fouad HA, FaroniLRDA, Tavares WS, Ribeiro RC, Freitas SS, Zanuncio JS. Botanical extracts of plants from the Brazilian cerrado for the integrated management of Sitotroga cerealella (Lepidoptera: Gelechiidae) in stored grain. Journalof Stored Product Research. 2014; 57:6-11.

Hosamani R. Neuroprotective efficacy of Bacopamonnieri against rotenone induced oxidative stress and neurotoxicity in Drosophila melanogaster. Neurotoxicology. 2009;30(6):977-985.

Llorens JV, Navarro JA, Martínez-Sebastián MJ, Baylies MK, Schneuwly S, Botella JA, Moltó MD. Causative role of oxidative stress in a Drosophila model of Friedreich ataxia. The FASEB Journal. 2007;21(2):333-344.

Hosamani R, Ramesh SR. Attenuation of rotenone-induced mitochondrial oxidative damage and neurotoxicty in Drosophila melanogaster supplemented with creatine. Neurochemical Research. 2010; 35(9):1402-1412.

Arking R, Wells RA. Genetic alteration of normal aging processes is responsible for extended longevity in Drosophila. Developmental genetics. 1990;11(2):141-148.

Linderman JA, Chambers MC, Gupta AS, Schneider DS. Infection-related declines in chill coma recovery and negative geotaxis in Drosophila melanogaster. PLoS One. 2012;7(9):41907.

Bartholomew NR, Burdett JM, Vanden Brooks JM, Quinlan MC, Call GB. Impaired climbing and flight behaviour in Drosophila melanogaster following carbon dioxide anesthesia. Scientific Reports. 2015;5: 15298.

Ng V, Koh D, Wee A, Chia SE. Salivary acetylcholinesterase as a biomarker for organophosphate exposure. Occupational Medicine. 2009;59(2):120-122.

Hernández AF, López O, Rodrigo L, Gil F, Pena G, Serrano JL, Pla A. Changes in erythrocyte enzymes in humans long-term exposed to pesticides: influence of several markers of individual susceptibility. Toxicology Letters. 2005;159(1):13-21.

Souza MS, Magnarelli GG, Rovedatti MG, Cruz SS, D'Angelo APD. Prenatal exposure to pesticides: analysis of human placental acetylcholinesterase, glutathione S-transferase and catalase as biomarkers of effect. Biomarkers. 2005;10(5):376-389.

Safi JM, Mourad TA, Yassin MM. Hematological biomarkers in farm workers exposed to organophosphorus pesticides in the Gaza Strip. Archives of Environmental & Occupational Health. 2005;60(5):235-241.

Simoniello MF, Kleinsorge EC, Scagnetti JA, Mastandrea C, Grigolato RA, Paonessa AM, Carballo MA. Biomarkers of cellular reaction to pesticide exposure in a rural population. Biomarkers. 2010;15(1): 52-60.

Kavitha P, Rao JV. Oxidative stress and locomotor behaviour response as biomarkers for assessing recovery status of mosquito fish, Gambusia affinis after lethal effect of an organophosphate pesticide, monocrotophos. Pesticide Biochemistry and Physiology. 2007;87(2): 182-188.

Sanchez‐Hernandez JC, Sanchez BM. Lizard cholinesterases as biomarkers of pesticide exposure: Enzymological characterization. Environmental Toxicology and Chemistry: An International Journal. 2002;21(11):2319-2325.

Agarwal A, Aponte-Mellado A, Premkumar BJ, Shaman A, Gupta S. The effects of oxidative stress on female reproduction: A review. Reproductive Biology and Endocrinology. 2012;10(1):49.

De Peyster A, Willis WO, Liebhaber M. Cholinesterase activity in pregnant women and newborns. Journal of Toxicology and Clinical Toxicology. 1994; 32(6):683– 96

McKean KA, Yourth CP, Lazzaro BP, Clark AG. The evolutionary costs of immunological maintenance and deployment. BMC Evolutionary Biology. 2008;8(1):76.

Linder JE, Promislow DE. Cross-generational fitness effects of infection in Drosophila melanogaster. Fly. 2009;3(2): 143-150.