Thermoregulation and Haematological Responses to Induced Acute Haemorrhage in Adult Nubian Goats

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Selma E. Abdalla
Abdalla M. Abdelatif
Shadia A. Omer


Background and Objectives: Acute haemorrhage is a major cause of morbidity and mortality in surgery and trauma due to ischaemic lesions followed by multiple organ dysfunction. After haemorrhage, compensatory redistribution of blood volume may occur to more vital and survival organs. The objective of the study was evaluation of thermoregulation and haematological responses to acute haemorrhage in Nubian goats.

Materials and Methods: The study used 6 female mature goats, weighing 18.0 kg on average. The goats were subjected to 40% bleeding. The rectal temperature (Tr), respiration rate (RR) and heart rate (HR) were monitored before bleeding and then immediately after bleeding and at 1,2,3,4,5,6,24 hrs post-bleeding. Venous blood samples were collected before and then immediately after bleeding and at 6, 24, 48, 72 hrs post-bleeding. The haemoglobin concentration (Hb), packed cell volume (PCV), total leukocyte count(TLC), differential leukocyte count (DLC), serum total protein, albumin, and concentrations of sodium (Na) were determined.

Results: The post-bleeding values of Tr, RR and HR were significantly higher compared to the pre-bleeding values. The post-bleeding values of PCV, Hb concentration, erythrocyte count and TLC were lower compared to prebleeding values. The ratios of lymphocytes, monocytes and eosinophils decreased, whereas the neutrophil ratio increased post-bleeding. The values of serum total protein, albumin and serum Na concentrations decreased post-bleeding.

Conclusion: The studies indicate that the goat can be adopted as a valid research model in investigations of experimental haemorrhage. The findings have implications in the fields of veterinary haematology and surgery and comparative medicine.

Goat, acute haemorrhage, thermoregulation, blood constituents

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How to Cite
Abdalla, S. E., Abdelatif, A. M., & Omer, S. A. (2019). Thermoregulation and Haematological Responses to Induced Acute Haemorrhage in Adult Nubian Goats. Journal of Applied Life Sciences International, 21(2), 1-12.
Original Research Article


Rhee P, Koustova E, Alam HB. Searching for the optimal resuscitation method: Recommendations for the initial fluid resuscitation of combat casualties. J. Trauma . 2003; 54:S52-S62.

Sharma P, Benford B, Karaian JE, Keneally R. Effects of volume and com-position of the resuscitative fluids in the treatment of haemorrhagic shock. J. Emerg. Trauma Shock. 2012;5:309.

Marino PL. Haemorrhage and hypovo-laemia. In: Marino PL, ed. The ICU Book. Philadelphia, Lippincott William and Wilkins. 2007;211-233.

Gutierrez G, Reines H, Wulf-Gutierrez ME. Clinical review: Haemorrhagic shock. Crit. Care, 2004;8:373-381 .

Dutton RP. Current concepts in haem-orrhagic shock. Anesthesiol. Clin. 2007; 25:23-34.

Abreu GF, Coelho AR, Aguiar JL, Moura Filho SA. Cardiocirculatory changes in haemorrhagic shock induced in pigs submitted to three distinct therapeutic methods. Acta Circ. Bras. 2010;25:126-130.

Pacagnella RC, Souza JP, Durocher J, Perel P, Blum J, Winikoff B, Gulmezoglu AM. A systematic review of the relationship between blood loss and clinical signs. PloS One. 2013;8:e57594.

Edward S. Shock: Types,classifications and exploration of their physiological effects. Emerg. Nurse. 2001;9:29-38.

Miyagatani Y, Yukioka T, Ohta S, Ohta S, Matsuda H, Shimazu H, Shimazaki S. Vascular tone in patients with haemorrhagic shock. J. Trauma. 1999;47: 282-287.

Rose JC, Block SM, Flowe K, Morris M, South S, Sundberg DK, Zimmerman C. Responses to converting-enzyme inhibition and haemorrhage in newborn lambs and adult sheep. Am. J. Physiol. 1987;252:R306-R313.

Douzinas EE, Andrianakis I, Livaditi O, Paneris P, Tasoulis M, Pelekanou A, Betrosian A, Giamarellos-Bourboulis EJ. The level of hypotension during haemor-rhagic shock is a major determinant of the post-resuscitation systemic inflammatory response: An experimental study. BMC Physiol. 2008;8:2483-989.

Sousa RS, Chaves DF, Barreto-Júnior RA, Sousa IKF, Soares HS, Barros IO, Minervino AHH, Ortolani EL. Clinical, haematological and biochemical responses of sheep undergoing autologous blood transfusion. BMC Veterinary Research. 2012;8:61.

Eichstaedt HC, Tamez D, Byler D, Nolden L, Inman R, Eya K, Cohen D, Frazier OH, Radovancevic B. Prolonged controlled haemorrhagic shock in large animals‐ potential for ventricular assist support? Asaio J. 2000;46:199.

Abdalla SE, Abdelatif AM. Effects of haemorrhage on thermoregulation, heart rate and blood constituents in goats (Capra hircus). Pak. J. Biol. l Sci. 2008;11:1194-1203.

Pirkle JC, Gann DS . Restitution of blood volume after haemorrhage: role of the adrenal cortex. Am. J. Physiol. 1976;230: 1683-1687.

Jain JC. Haematologic techniqes In: Schalms veterinary haematology. 4th Edition. Lea and Febiger, Philadelphia, USA, 1986;20-86.

Wootton IDP. Plasma sodium and potassium. In; Micro-analysis in medical biochemistry. 5th Edition. Churchill limited. London. 1974;62-65.

SAS. SAS/STAT Users Guide, Release 6.03Edn: SAS Institute, Inc. Cary, N; 1998.

Vatner SF. Effect of haemorrhage on regional blood flow distribution in dog and primates. J. Clin. Invest. 1974;54:225-235.

Abdelatif A, Abdalla S. Physiological responses of goats (Capra hircus) to haemorrhage as influenced by age. Global Veterinaria. 2009;3:151-164.

Abdalla SE, Abdellatif AM. Physiological responses of goats (Capra hircus) to haemorrhage as Influenced by splenectomy. American-Eurasian J. Sci. Res. 2010;5:76-87.

Brown JW, Whitehurst ME, Gordon CJ, Carroll RG. Thermoregulatory set point decreases after haemorrhage in rats. Shock. 2005;23:239-242.

Henderson RA, Whitehurst ME, Morgan KR, Carroll RG. Reduced metabolic rate accompanies the haemorrhage-induced hypothermia in conscious rats. Resusci-tation. 2000;44:129-138.

Fontelles MJ, Carvalho RMD, Dolivire LMR, Madeira A, Borges PVG, Dolivire MS. Profile of haemodynamic and gasometric parameters in rabbits submitted to controlled haemorrhagic shock. Revista Paraense de Medicina . 2007;21:15-21.

Devar D, Moore FA, Moore EE, Balogh Z. Post-injury multiple organ failure. Injury. 2009;40:912-918.

Maltz E, Olsson K, Glick S, Fyhrqist F, Silanikove N, Choshniak I, Shkolnik A. Homeostatic responses to water depriva-tion or haemorrhage in lactating and non-lactating Bedouin goats. Comp. Biochem. Physiol. A – Comp. Physiol. 1984;77:79-84.

Jochem J. Haematological, blood gas and acid-base effects of central histamine-induced reversal of critical haemorrhagic hypotension in rats. J. Physiol. Pharmacol. 2001;52:447-458 .

Jochem J, Josko J, Gwozdz B. Endo-genous opioid peptides system in haemorrhagic shock-central cardiovascular regulation. Med. Sci. Momit. 2001;7:545-549.

Guyton AC, Hall JE. Textbook of medical physioloy . 10th Edition. Saunders, Elsevier, Philadelphia; 2002.

Wintour EM, Moritz LM, Potocnick SJ. Cardiovascular, hormonal and metabolic responses to severe prolonged haemorrhage in adult sheep. Am. J. Vet. Res. 1995;56:1232-1240.

Schadt JC, Ludbrook J. Haemodynamic and neurohumoral responses to acute hypovolaemia in conscious mammals. Am. J. Physiol. 1991;260(2 Pt 2):H305-18.

Reece WOI. The Kidneys. In: Duckes’ Physiology of Domestic Animals. 11th Edition. (Editors: Swenson M, Reece WO). Cornell University Press, Ithaca and London. 1993;596.

Maier RV. Pathogenesis of multiple organ dysfunction syndrome: Endotoxin, inflammatory cells, and their mediators: Cytokines and reactive oxygen species. Surg. Infect. (Larchmt). 2000;1:197-205.

Duncan JR, Prasse KW, Mahaffey EA. Veterinary Laboratory Medicine: Clinical Pathology. 3rd Edition, Iowa State University Press, Ames, Iowa; 1994.

Argolo EP, Firmino PR, Soares JO, Numens TL, Abrantes MR, et al. Clinical responses to acute blood loss in goats. Semina: Ciencias Agrarias (Londrina). 2018;39:583-592.

Swenson MJ. Physiological properties and cellular and chemical constituents of blood. In: Duckes Physiology of Domestic Animals,(Editors: Swenson M, Reece WO). 11th Edn., Cornell University Press. Ithaca and London. 1993;22-46.

Tyan ML. Effect of age on the intrinsic regulation of murine haemopoiesis. Mech. Ageing Dev. 1982;19:15-20.

Shatney C, Read G, Cuevo R, Formeister JF. The natural leukocyte response to haemorrhagic shock. Adv. Shock Res. 1981;5:79-88.

Megdasian KG. Therapeutics in practice: Acute blood loss. Compendium Equine. 2008;3:80-90.

Block SM, Pixley JE, Wray AH, Ray D, Barnes KD, Engstrom PC, Rose JC. Blood volume restitution after haemorrhage in newborn lambs. Am. J. Physiol. 1989;258: R647-R652.

Ikomi F, Kawai Y, Ohhashi T. Recent advances in lymph dynamic analysis in lymphatics and lymph nodes . Ann. Vasc. Dis. 2012;5:258-268.

Hjelmqvist H, Ullman J, Gunnarsson U, Lundberg JM, Rungren M, Haemodynamic and humoral responses to repeated hypotensive haemorrhage in conscious sheep. Acta Physiol. Scand. 1991;143(1): 55-64.