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Aims: The present study aimed to investigate antidiarrheal and thrombolytic effect of ethanolic extract of leaves of A. dealbatum in mice.
Study design: Antidiarrheal effect was evaluated by castor oil-induced diarrhea method at two different concentrations in mice and in vitro thrombolytic activity was analyzed with clot lysis assay of human blood.
Place and duration of study: Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh, between December 2018 and February 2019.
Methodology: The male Swiss mice’s were divided into four groups (n = 5). First group was orally treated with 1% Tween-80 (10 ml/kg) and second group was orally treated with loperamide (5 mg/kg). Third and fourth group were orally treated with ethanolic extract of leaves of A. dealbatum at 200 and 400 mg/kg accordingly. Human RBCs were collected for conducting thrombolytic assay. During this study, 1.5 ml of venous blood was drawn from healthy volunteers (n = 10) and Streptokinase was employed as positive control and distilled water was employed as negative control.
Results: In castor oil induced diarrhea model, ethanolic extract of leaves of A. dealbatum at 200, 400 mg/kg and loperamide (5 mg/kg) significantly reduced the number of feces and increase percent of inhibition of defecations compared to negative control. The extract showed percent of inhibition of defecation of 16.67 and 37.50 for 200 and 400 mg/ml respectively where the positive control loperamide showed 66.67%. Percentage of clot disruptions were 4.51 (p<.001), 75.69 (p<.001) and 26.07 (p<.001) for water, streptokinase and 10 mg/ml extract respectively.
Conclusion: Based on the results from in vivo and in vitro activities, the leaves of A. dealbatum were found to be a potential source of new antidiarrheal and thrombolytic agents.
Keshari P. Pradeep. A review of conservation and sustainable use of medicinal plant with special reference of Tecomella undulata (Sm.) Seem. Journal of Pharmacognosy and Phytochemistry. 2018;3:09-13.
Akinmoladun AC, et al. Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum gratissimum. Scientific Research and Essay. 2007;2(5):163-166.
Phuaklee P, Sakpakdeejaroen I, Itharat A. Cytotoxic and antioxidant activities of two species of ginger extracts. Thai Journal of Pharmacology. 2010;32(1):82-85.
Taylor L, Taylor TN, Krings M. Paleo-botany: The Biology and evolution of fossil plants. 2nd ed. Academic Press; 2009.
Fern K. Useful Tropical Plants; 2014.
Uddin SB. Bangladesh ethnobotany online database. Department of Botany, Chittagong University: Chittagong 4331, Bangladesh; 2012.
Ezekwesili CN, Obiora KA, Ugwu OP. Evaluation of anti-diarrhoeal property of crude aqueous extract of Ocimum gratissimum L. (Labiatae) in rats. Nigerian Society for Experimental Biology. 2004; 16(2):122-131.
Birru EM, et al. Antidiarrheal activity of crude methanolic root extract of Idigofera spicata Forssk.(Fabaceae). BMC Comple-ment Altern Med. 2016;16:272.
Kabir MS, et al. Antioxidant, antidiarrheal, hypoglycemic and thrombolytic activities of organic and aqueous extracts of Hopea odorata leaves and in silico PASS prediction of its isolated compounds. BMC Complement Altern Med. 2016;16(1):474.
Nigam V, Paarakh PM. Evaluation of anti-diarrhoeal activity of hydro alcoholic extract of chenopodium album L. Indian Journal of Natural Products and Resources 4, 61-66., Evaluation of anti-diarrhoeal activity of hydro alcoholic extract of Chenopodium album L. Indian Journal of Natural Products and Resources. 2013;4(1):61-66.
Shoba FG, Thomas M. Evaluation of anti-diarrhoeal effect of four medicinal plants on castor oilinduced gastrointestinal motility in mice. Advances in Applied Science Research. 2014;5(4):153-156.
Degu A, Engidawork E, Shibeshi W. Evaluation of the anti-diarrheal activity of the leaf extract of Croton macrostachyus Hocsht. ex Del. (Euphorbiaceae) in mice model. BMC Complement Altern Med. 2016;16(1):379.
Konaté K, et al. Antidiarrheal and anti-microbial profiles extracts of the leaves from Trichilia emetica Vahl. (Meliaceae). Asian Pacific Journal of Tropical Biomedicine. 2015;5(3):242-248.
Abdel-Razik A, et al. De-novo portal vein thrombosis in liver cirrhosis: Risk factors and correlation with the model for end-stage liver disease scoring system. Eur J Gastroenterol Hepatol. 2015;27(5):585-92.
Qin J, et al. A panel of microRNAs as a new biomarkers for the detection of deep vein thrombosis. J Thromb Thrombolysis. 2015;39(2):215-21.
Mucklow JC. Thrombolytic treatment. Streptokinase is more economical than alteplase. BMJ. 1995;311(7018):1506.
Naderi GA, et al. Fibrinolytic effects of Ginkgo biloba extract. Exp Clin Cardiol. 2005;10(2):85-7.
Rahman MA, et al. Effects of organic extracts of six Bangladeshi plants on in vitro thrombolysis and cytotoxicity. BMC Complement Altern Med. 2013;13:25.
Dragstedt LR. Ethical considerations in the use and care of laboratory animals. J Med Educ. 1960;35:1-3.
Franca CS, et al. Analgesic and antidiarrheal properties of Ocimum selloi essential oil in mice. Fitoterapia. 2008; 79(7-8):569-73.
Abdullahi M, et al. Medicinal and economic plants of Nupeland. Bida, Nigeria: Jube Evans Books and Publications; 2003.
Prasad S, et al. Development of an in vitro model to study clot lysis activity of thrombolytic drugs. Thromb J. 2006;4: 14.
Kawsar MH, et al. Studies of thrombolytic and cytotoxic properties of two asteraceous plants of Bangladesh. Bangladesh Pharmaceutical Journal. 2011; 14(2):103-106.
Agbor GA, Leopold T, Jeanne NY. The antidiarrhoeal activity of Alchornea cordifolia leaf extract. Phytother Res. 2004; 18(11):873-6.
Umer S, Tekewe A, Kebede N. Antidiarrhoeal and antimicrobial activity of Calpurnia aurea leaf extract. BMC Complement Altern Med. 2013;13:21.
Racusen LC, Binder HJ. Ricinoleic acid stimulation of active anion secretion in colonic mucosa of the rat. J Clin Invest. 1979;63(4):743-9.
Pinto A, et al. Time course of PAF formation by gastrointestinal tissue in rats after castor oil challenge. J Pharm Pharmacol. 1992;44(3):224-6.
Mascolo N, et al. Nitric oxide and castor oil-induced diarrhea. J Pharmacol Exp Ther. 1994;268(1):291-5.
Capasso F, et al. Dissociation of castor oil-induced diarrhoea and intestinal mucosal injury in rat: Effect of NG-nitro-L-arginine methyl ester. Br J Pharmacol. 1994;113(4): 1127-30.
Zafar Imam M, Sultana S, Akter S. Anti-nociceptive, antidiarrheal, and neuro-pharmacological activities of Barringtonia acutangula. Pharm Biol. 2012;50(9):1078-84.
Shah S. Evaluation of diarrhea: The challenge continues! Part-I. Indian J Med Sci. 2004;58(2):75-8.
Chitme HR, Chandra M, Kaushik S. Studies on anti-diarrhoeal activity of Calotropis gigantea R.Br. in experimental animals. J Pharm Pharm Sci. 2004;7(1): 70-5.
Dosso K, et al. Antidiarrhoeal activity of an ethanol extract of the stem bark of Piliostigma reticulatum (Caesalpiniaceae) in rats. Afr J Tradit Complement Altern Med. 2012;9(2):242-9.
Di Carlo G, et al. Inhibition of intestinal motility and secretion by flavonoids in mice and rats: Structure-activity relationships. J Pharm Pharmacol. 1993;45(12):1054-9.
Brijesh S, et al. Studies on the antidiarrhoeal activity of Aegle marmelos unripe fruit: validating its traditional usage. BMC Complement Altern Med. 2009;9:47.
Palta S, Saroa R, Palta A. Overview of the coagulation system. Indian J Anaesth. 2014;58(5):515-23.
Das A, et al. Investigation of in vitro thrombolytic potential of ethanolic extract of Momordica charantia fruits: An anti-diabetic medicinal plant. Der Pharmacia Sinica. 2013;4:104-108.
Kumar Verma S, Jain V, Singh DP. Effect of greater cardamom (Amomum subulatum Roxb.) on blood lipids, fibrinolysis and total antioxidant status in patients with ischemic heart disease. Asian Pacific Journal of Tropical Disease. 2012;2(2):739-743.
Ali MR, et al. Preliminary phytochemical screening and in vitro thrombolytic potential of the methanolic extract of enhydra fluctuans lour (Leaves). International Journal of Pharmamedix India. 2013;1(2):270-280.