Antioxidant Status of Breast, Cervical and Ovarian Cancer Patients at Various Menopausal Stages in Lagos State, Nigeria

Main Article Content

E. I. Ayo
M. F. Asaolu
O. G. Oyebanji
I. Akinlua
A. A. Sonuga

Abstract

Cancer is a collection of diseases which involves the abnormal growth of cells with the potential to invade or spread to other parts of the body. The aim of this study is to access the antioxidant status of women with female predominant cancer (breast, cervical and ovarian) in relationship with their menopausal stages. Blood samples were collected from 180 freshly diagnosed female patients of breast, cervical and ovarian cancer at Lagos State University Teaching Hospital, Idi- Araba, Mushin, Lagos and Lagos State University Teaching Hospital Ikeja, Lagos and 60 relatively healthy subjects at different menopausal stages. Serum catalase (CAT), Superoxide dismutase (SOD) activites, Reduced Glutathione (GSH), Vitamin C and E concentrations were evaluated in subject’s blood sample using standard established methods. The results obtained were subjected to statistical analysis (p<0.05). However, the results of the female cancer patients at each menopausal stage were compared to premenopausal, menopausal and postmenopausal control groups, while the results obtained from the menopausal and postmenopausal control subjects were compared to the premenopausal control subjects. There was significant decrease (p<0.05) in the activities of CAT, SOD and in the concentrations of GSH, Vitamins C and E in all the menopausal stages when compared to the control groups. However, MDA concentrations showed significant increase (p<0.05) in all the menopausal stages in comparison to the corresponding control groups. These findings suggest that cancer patients might be at risk from oxidative cell damage. Therefore, further research is required in this field with a view of improving the management of cancers predominant in females.

Keywords:
Cancer, catalase, superoxide dismutase, reduced glutathione, Vitamin C and E

Article Details

How to Cite
Ayo, E. I., Asaolu, M. F., Oyebanji, O. G., Akinlua, I., & Sonuga, A. A. (2019). Antioxidant Status of Breast, Cervical and Ovarian Cancer Patients at Various Menopausal Stages in Lagos State, Nigeria. Journal of Applied Life Sciences International, 20(4), 1-7. https://doi.org/10.9734/jalsi/2019/v20i430092
Section
Original Research Article

References

National Cancer Institute. What is cancer? (PDQ®); 2014.
Available:https://www.cancer.gov/types/ovarian/hp/ovarianpreventionpdq#section/all?redirect=true
[Accessed 8th April 2019]

World Health Organization, Fact sheet: Cancer; 2018.
Available:https://www.who.int/news-room/fact-sheets/detail/cancer
[Accessed 8th May, 2019]

World Health Organization, Fact sheet No. 297: Cancer; 2014.
Available:https://www.who.int/en/news-room/fact-sheets/detail/cancer
[Accessed 8th April 2019]

Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. Ca cancer J Clin. 2011;61(2): 69–90.

William BC, Tara CR. Molecular pathology: The molecular basis of human disease. Amsterdam: Elsevier Academic Press. 2009;66.

Jayasekara H, MacInnis RJ, Room R, English DR. Long-term alcohol consumption and breast, upper aero-digestive tract and colorectal cancer risk: A systematic review and meta-analysis. Alcohol Alcohol. 2016;51(3): 315–330.

Anand P, Kunnumakkara AB, Kunnumakara AB, Sundaram C, Harikumar KB, Tharakan ST, Lai OS, Sung B, Aggarwal BB. Cancer is a preventable disease that requires major lifestyle changes. Pharma Res. 2008;25(9):2097–2116.

Harlow SD, Gass M, Hall JE, Lobo R, Maki P, Rebar RW, Sherman S, Sluss PM, de Villiers TJ. Executive summary of the Stages of Reproductive Aging Workshop +10: Addressing the unfinished agenda of staging reproductive aging. Fertil Steril. 2012;97(4):398–406.

Ohkawa H, Ohishi N, Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analy Biochem. 1979;95(2):351-358.

Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974;47(3):469-74.

Beutler E, Kelly BM. The effect of sodium nitrite on red cell GSH. Experientia. 1963; 19:96-97.

Beers RF, Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem. 1952;195(1):133-140.

Omaye ST, Turnbull TD, Sauberlich HC. Selected method for the determination of ascorbic acid in animal cells tissues and fluids. Methods Enzymol. 1979;62:3-11.

Rutkowski M, Grzegorczyk K. Modifi-cations of spectrophotometric methods for antioxidative vitamins determination convenient in analytic practice. Acta Scien Polo Techn Alim. 2007;6(3):17-28.

Kumaraguruparan R, Subapriya R, Kabalimoorthy J, Nagini S. Antioxidant profile in the circulation of patients with fibroadenoma and adenocarcinoma of the breast. Clin Biochem. 2002;35(4):275- 79.

Singh RK, Bhattacharya SK, Acharya SB. Pharmacological activity of Abies pindrow. J Ethnopharmacol. 2000;73:47–51.

Gönenç A, Tokgöz D, Aslan S, Torun M. Oxidative stress in relation to lipid profiles in different stages of breast cancer. Indian J Biochem Biophys. 2005;42:190–194.

Sánchez-Rodríguez MA, Zacarías-Flores M, Mendoza-Núñez VM. Menopause and Oxidative Stress. Research Gate. 2015; 32-49.