In vitro Antioxidant Activity and Inhibition of Fe2+ and SNP Lipid Peroxidation of African Mistletoes (Tapinanthus globiferus) from Three Selected Host Plants in Jos Plateau State Nigeria

Main Article Content

Jane-Rose I. Oche
Titilayo O. Johnson
Augustina O. Akinsanmi
Kiri H. Jaryum
Timothy Francis


Aim: The aim of this study was to investigate and compare the antioxidative properties of the mistletoe plant obtained from three different host species namely Psidium guajava, Vernonia amygdalina and Moringa olifera lam.

Study Design: Experimental Design

Place and Duration of Study: Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences and Department of Biochemistry, College of Health Sciences, University of Jos, Nigeria.

Methodology: Crude methanolic leaf extracts were studied for their antioxidative properties; Iron reducing and Iron-chelating activities, Nitric oxide (NO) radical and 2,2-diphenyl 1-picrylhydrazyl (DPPH) radical scavenging activities and the lipid peroxidation and thiobarbituric acid reaction (TBAR) methods. One way ANOVA was used for the result analysis with P<.05 for significant difference.

Results: Mistletoes from Psidum guajava (MSPG) had significantly higher reducing property (0.16 – 0.20mg/mL); the chelating property of Mistletoes from Moringa olifera (MSMO) was significantly lower (45.7 – 58.9%); DPPH radical scavenging activity had no significant difference; and Nitric oxide scavenging activity was significantly higher in MSPG (72.1% in 75mg/mL) than the extracts from other hosts. MSPG had significantly higher TBAR inhibition using both FeSO4 (77.8% at 125µg/mL) and Sodium nitroprusside (61.6+1.0% at 125µg/mL) with an IC50 of 30.27µg/mL . Extract of Tapinanthus globiferus leaves from Psidium guajava had more antioxidative activities in the TBARs followed by Tapinanthus globiferus leaf extract from Vernonia amygdalina (MSVA).

Conclusion: From the study, mistletoes from Psidium guajava had higher antioxidant activity compared to other hosts, which probably justifies its use for treatment of cancer in traditional medicinal practice.

Antioxidant, traditional medicine, mistletoe, oxidative stress

Article Details

How to Cite
Oche, J.-R., Johnson, T., Akinsanmi, A., Jaryum, K., & Francis, T. (2019). In vitro Antioxidant Activity and Inhibition of Fe2+ and SNP Lipid Peroxidation of African Mistletoes (Tapinanthus globiferus) from Three Selected Host Plants in Jos Plateau State Nigeria. Journal of Applied Life Sciences International, 20(4), 1-10.
Original Research Article


Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A. Oxidative stress: Harms and Benefits for Human Health. Oxidative Medicine and Cellular Longevity. Review Article. 2017;13.
Article ID 8416763. Available:

Singh A, Singh S, Prasad AM. Role of Medicinal Plants for health perspective: Special Reference to Antioxidant Potential. J Chem Biol Ther. 2016;1(2):106.
DOI: 10.4172/2572-0406.1000106.

Adesina SK, Illoh HC, Imoh J, Imoh EJ. Ethnopharmacology, Chemistry and Medicinal Values: An Update. African Journal of Traditional, Complementary and Alternative Medicines (AJTCAM). 2013; 10(4):161-170.

Ogunmefun OT, Olatunji BP, Adarabioyo MI. Ethnomedicinal Survey on the Uses of Mistletoe in South-western Nigeria. European Journal of Medicinal Plants. 2015;8(4):224–230.
ISSN: 2231-0894.

Mohammed SZ, Eldur BZ, Abbashar AA. Distribution and host range of loranthaceae (Tapinanthus globofenis (A.Rich) Van Teigh) along the nile banks in central sudan. International Journal of scientific and Technology Research. 2014;3:3.
ISSN: 2277-8616.

Ibrahim JA, Egharevba HO, Jegede AI, Ugbabe GE, Muazzam I, Kunle OF, Gamaniel KS. Medicinal plants used and the perception of plant endangerment by the traditional medicine practitioners of Nasarawa State, Nigeria: A pilot study International Journal of Biodiversity and Conservation. 2016;8(1):8-20.
DOI: 10.5897/IJBC2015.0842 Article Number: BD3333F56808 ISSN 2141-243X

Shrikant N. 10 Health Benefits of Mistletoe; 2016.

(Retrieved from on 3rd January, 2017)

Chu Y, Sun J, Wu X, Liu R. Antioxidant and antiproliferative activities of common vegetables. Journal of Agricultural and Food Chemistry. 2002;49(7):3216–3222.

Sofowora A. Medicinal plants and traditional medicines in Africa. Spectrum books Ltd Ibadan Nig. 1993;289.

Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis; 1973.
ISBN: 13:978-0-412-23050-9

e-ISBN13:978-94-009-5921-7 DOI:10.1007/978-94-009-5921-7

Trease GE, Evans WC. Textbook of Pharmacognosy, 12th Edition Tindall and Co., London. 1983;343–383.

Oyaizu M. Studies on products of browning reaction: Antioxidant activity of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition. 1986;44: 307–315.

Minotti G, Aust SD. An investigation into the mechanism of citrate – Fe3+-dependent lipid peroxidation. Free Radical Biology and Medicine Journal. 1987;3:379–387.

Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L. The nitric oxide – scavenging properties of Ginkgbo biloba extract EGb.761. Biochemical and Biophysical Research Communications. 1994;201: 748-755.

Gyamfi MA, Yonamine M, Aniya Y. Free radical scavenging action of medicinal herbs from Ghana: Thonninga sanguine in experimentally induced liver injuries. Gen Pharm. 1999;32:661–667.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry. 1979;95(2):351–358.

Dong-Pin X, Ya L, Xiao M, Tong Z, Yue Z, Jie Z, Jiao-Jiao Z, Hua-Bin L. Natural Antioxidants in Foods and Medicinal Plants: Extraction, Assessment and Resources. Int J Mol Sci. 2017;18(1):96.
DOI: 10.3390/ijms18010096
PMCID: PMC5297730
PMID: 28067795

Yu-Jie Z, Ren-You G, Sha L, Yue Z, An-NA L, Dong-Ping X, Hua-Bin L. Antioxidant Phytochemicals for the Prevention and Treatment of Chronic Diseases. Molecules. 2015;20:21138-21156.

Mattera R, Benvenuto M, Giganti MG, Tresoldi I, Pluchinotta FR, Bergante S, Tettamanti G, Masuelli L, Manzari V, Modesti A, Bei R. Effects of Polyphenols on Oxidative Stress – Mediated Injury in Cardiomyocytes. Review. Nutrient. 2017; 209(5).
DOI: 10.3390/nu9050523

Ogunbolude Y, Ibrahim M, Elekofehiniti O, Adeniran A, Abolaji AO, Rocha JB, Kandem JP. Effects of Tapinanthus globiferus and Zanthoxylum zanthoxyloid extract on human leukocytes in vitro. Journal of International Ethnopharmacology. 2014;3(4):167-72.

Adekunle AS, Aline AB, Afolabi OK, Rocha JB. Determination of free phenolic acids, flavonoid contents and antioxidant capacity of ethanolic extracts obtained from leaves of mistletoes (Tapinanthus globiferus) Asian J Pharm Clin Res. 2012;5:36-41.

Alam N, Bristi NJ, Rafiquzzaman. Review of on in-vivo and in-vitro methods evaluation of antioxidant activity. Saudi International Journal. 2013;21:143-152.
DOI: 10.1016/j.jsps.2012.05.02

Boora F, Chirisa E, Mukanganyama S. Evaluation of Nitrite Radical Scavenging Properties of Selected Zimbabwean Plant Extracts and their Phytoconstituents. Journal of Food Processing. 2014;7.

Article ID 918018, Available:

Yuste JE, Tarragon E, Campuzano CM, Ros-Bernai F. Implications of glial nitric oxide in neurodegenerative diseases. Font. Cell. Neurosci. 2015;9”32.
DOI: 10.3389/fncel.2015.0032

Kasote MD, Katyare SS, Hegde MV, Bae H. Significance of Antioxidative potentials of Plants and its relevance to Therapeutic alications. International Journal of Biological Sciences. 2015;11(8):982–9911.
PMCID: PMC4495415

Emmanuel HM, Gusau HL, Gidado LM, Ali B, Ugwah-Oguejiofor CJ, Yahaya A. Comparative Studies on Phytochemical and Antioxidant activities of Tapinanthus globiferus Piliostigma thonningii (A. Rich) and its host plant (Schum). Advance Pharmaceutical Journal. 2017;2(5):179-184.

Guiseppina B. Oxidative Stress and Lipid Peroxidation products in Cancer Progression and Therapy. Review ISRN Oncology; 2012. 21.
Article ID:137289 Available:

Prisacaru AE. Effect of antioxidants on polyunsaturated fatty acids – review. Acta Sci. Pol. Technol. Aliment. 2016;15(2): 121–129.
DOI: 10.17306/J.AFS.2016.2.12

Adedayo OA, Ganiyu O, Opeyemi BO, Funmilayo MO. A comparative study on antihypertensive and antioxidant properties of phenolic extracts from fruit and leaf of some guava (Psidium guajava L.) varieties. Comp Clin Pathol. 2016;25:363. Available: