Biofilm and Biosurfactant Mediated Aromatic Hydrocarbons Degradation by Marine Bacteria Isolated from Contaminated Marine Environments of Niger Delta

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Bright Obidinma Uba
Edna Ifeoma Chukwura
Ebere Linda Okoye
Amala Amechi Umebosi
Uchechukwu Fidelia Agbapulonwu
Olisaemeka Charles Muogbo
Chidiogo Leticia Okoye
Lilian Onyinyechukwu Oranta
Adaeze Mercy Odunukwe
Chinonso Peace Ndurue
Ogechi Salvation Ehirim

Abstract

Aims: To examine the effects of biofilm and biosurfactant associated cells of marine bacteria isolated from contaminated marine environments of Niger Delta on aromatic hydrocarbon degradation.

Study Design: Nine treatments and the controls designs were set up in triplicates containing 100 mL of sterile modified mineral basal medium in 250 mL conical flasks supplemented with 50, 100, 200 and 300 ppm of xylene, anthracene and pyrene each and nine marine hydrocarbon degraders; incubated at 24°C for 5 - 7 days. The nine treatments and control set ups designated as ANT1, XYL2, PYR3, ANT4, PYR5, ANT6, XYL7, XYL8, PYR9 and CTRL (without hydrocarbons) were used to examine the effects of biofilm and biosurfactant produced by the marine bacteria on aromatic hydrocarbon degradability.

Place and Duration of Study: Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli Nigeria between September, 2014 and August, 2017.

Methodology: A laboratory scale study was carried on six composite samples of the sediment and water samples from the three studied areas using enrichment, selection, morphological, biochemical, growth effect, emulsification, surface tension, bacterial adherence to hydrocarbon (BATH), salt aggregation (SAT) and microtitre plate biofilm formation tests.

Results: The findings revealed that the three sampling sites harbour a lot of efficient aromatic degrading bacterial strains belonging to the genera: Providencia, Alcaligenes, Brevundimonas, Myroides, Serratia, and Bacillus able to significantly (P = .05) degrade the aromatic hydrocarbons. Significant positive correlation between biofilm formation and emulsification activity (r = 0.670; P = .05), was observed while surface tension (r = 0.134; P > .05), BATH (r = 0.142; P > .05) and SAT (r = 0.227; P > .05) had no significant positive correlation with biofilm formation.

Conclusion: Thus, the metabolic traits potentials of these strains could be exploited for in situ bioremediation intervention in the coastal areas of Nigeria.

Keywords:
Aromatic hydrocarbons, aquatic pollution, bioremediation, biosurfactants, biofilm formation marine bacteria, Niger Delta

Article Details

How to Cite
Uba, B., Chukwura, E., Okoye, E., Umebosi, A., Agbapulonwu, U., Muogbo, O., Okoye, C., Oranta, L., Odunukwe, A., Ndurue, C., & Ehirim, O. (2019). Biofilm and Biosurfactant Mediated Aromatic Hydrocarbons Degradation by Marine Bacteria Isolated from Contaminated Marine Environments of Niger Delta. Journal of Applied Life Sciences International, 19(4), 1-17. https://doi.org/10.9734/jalsi/2018/v19i430069
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Original Research Article