Anti-ulcerogenic, Antioxidant and Mucogenic Effects of L-cysteine in Gastric Tissue of Wistar Rats

Main Article Content

Oluwadamilola Victor Ajayi
Francis S. Oluwole
Ayobami Dare

Abstract

Aim: To evaluate the effect of L-cysteine pretreatment on indomethacin induced ulceration in male wistar rats.

Study Design: Experimental animal study.

Place and Duration of Study: Department of Physiology (inflammation and Gastrointestinal secretion unit), College of Medicine, University of Ibadan, Nigeria, between January and July 2017.

Methodology: Fifty male wistar rats were used for this study and were randomly divided into two study groups of twenty-five (25) animals each. The first sub-group was used for the anti-ulcer studies; antioxidant enzymes (SOD and MDA), Nitric oxide (NO), mean ulcer score and gastric blood flow (GBF), while the second sub-group was used for the gastric mucus secretion study. Each sub group was divided into five groups with five animals per group as follows: ulcer control, L-cysteine (100 mg/kg, 300 mg/kg and 500 mg/kg), cimetidine (50 mg/kg). Results were analysed using ANOVA and reported as Mean ± SEM. Values were considered significant at P<0.05.

Results: The results of this study showed that L-cysteine (100 mg, 300 mg, and 500 mg respectively) pretreatment significantly reduced mean ulcer score (9.5±1.9; 7.5±1.5; 4.5±0.9) and MDA level (7.2±0.23; 7.49±0.3; 6.54±0.55), and increased SOD activity (10.69±0.1; 10.12±0.29; 14.76±0.07) when compared with the mean ulcer score, MDA and SOD in the ulcer control group (39.5±7.9; 10.62±1.11; 5.02±0.74). Also, NO level (10.8±0.44; 10.37±0.18; 8.41±0.06), gastric mucus secretion (0.92±0.008; 0.94±0.001; 0.99±0.001) and GBF (2.08±0.02; 2.11±0.06; 2.11±0.01) were significantly (p<0.05) higher in the L-cysteine pre-treated animals when compared with NO, mucus secretion and GBF in the ulcer control (7.86±0.09; 0.82±0.01).

Conclusion: This study shows that L-cysteine pre-treatment has anti-ulcer potential which might be mediated through increased antioxidant enzymes, increased mucus secretion and enhancing gastric blood flow. This will be of immense advantage in the treatment of peptic ulcer.

Keywords:
L-cysteine, cimetidine, antioxidants, mucus secretion, anti-ulcer.

Article Details

How to Cite
Ajayi, O., S. Oluwole, F., & Dare, A. (2018). Anti-ulcerogenic, Antioxidant and Mucogenic Effects of L-cysteine in Gastric Tissue of Wistar Rats. Journal of Applied Life Sciences International, 19(3), 1-10. https://doi.org/10.9734/JALSI/2018/45289
Section
Original Research Article