Improvement of the Efficacy an WHO Alcohol-based Hand Rub Formulation by Reducing Isopropanol Content and Associating Syzygium aromarticum and Piper nigrum Essential Oils

Main Article Content

Mahamat Ismail
Veronique Penlap
Wilfred F. Mbacham
Sylvain Leroy Sado Kamdem

Abstract

Aim: This work was carried out in order to reduce the isopropanol contain of a World Health Organization (WHO) basic alcohol based hand rub (ABHR) formulation by substituting part of it with ethanol and essential oils.


Study Design: A quasi-experimental design was used, based on WHO basic formulation modification, antimicrobial assessment followed by a panel test and challenge test of the best formulation.


Place and Duration of the Study: The study was carried out in different laboratory of the University of Yaoundé 1 during March 2017 to July 2018.


Methodology: This study was performed by producing different formulations nested from the WHO basic formulation and testing their antimicrobial capacity on selected strains based on the microbial percentage reduction. Following this, the best formulation was compared to two commercial products trough a panel test and later challenged with selected organism (Staphylococcus aureus SR196, Salmonella Typhi 15SA, Escherichia coli ATTC25922 and Pseudomonas aeruginosa PA01) inoculated in the product after different storage periods for one year and the antimicrobial stability assessed on the same strain for the same length of conservation.


Results: The partial substitution of isopropanol with ethanol and Piper nigrum and Syzygium aromaticum essential oils proved to possess more antimicrobial properties than the original WHO formulation. The best formulation caused a 6 Log cells/ml reduction of the initial population compared to the 4 Log cells/ml of the WHO formulation. The product also proved to maintain its activity for one year and to be able to deactivate possible contaminations by Salmonella Typhi 15SA, Staphylococcus aureus NCTC10652, Escherichia coli ATCC25922, Pseudomonas aeruginosa PA01.


Conclusion: The present work is a contribution to the improvement of ABHRs and could permit the reduction of hand hygiene associated infections in industries and health care facilities.

Keywords:
Hand rub formulation, isopropanol content, Syzygium aromarticum, Piper nigrum, essential oils

Article Details

How to Cite
Ismail, M., Penlap, V., Mbacham, W. F., & Kamdem, S. L. S. (2019). Improvement of the Efficacy an WHO Alcohol-based Hand Rub Formulation by Reducing Isopropanol Content and Associating Syzygium aromarticum and Piper nigrum Essential Oils. Journal of Applied Life Sciences International, 21(3), 1-11. https://doi.org/10.9734/jalsi/2019/v21i330105
Section
Original Research Article

References

WHO. Guidelines on hand hygiene in health CARE. Organisation mondiale de la santé, Août; 2009. Disponible sur.
Available:http://whqlibdoc.who.int/publications/2009/9789241597906_eng.pdf vailable to 10‐10‐2010

Vincent JL. Nosocomial infections in adult intensive-care units. Lancet. 2003;3(61): 2068-2077.

World alliance for patient safety. The Global patient safety challenge 2005 2006. Clean Care is Safer Care. Genev a, World Health Organization; 2005.

Hui KL, Hishamuddin AH, Kwai LT, Lay CC. Assessment of food safety knowledge, attitude, self-reported practices and microbiological hand hygiene of food handlers. Int. J. Environ. Res. Public Health. 2017;14:55.

Michaels B, Keller C, Belvins M, Paoli G, Ruthman T, Todd E, Griffith CJ. Prevention of food worker transmission of foodborne pathogens: Risk assessment and evaluation of effective hygiene intervention strategies. Food Serv. Technol. 2004;4: 31–49.

Boyce JM, Pittet D. Guideline for hand hygiene in health-care settings. Recommendations of the healthcare infection control practices advisory committee and the HICPAC/SHEA/APIC/IDSA Hand Hygiene Task Force. Morb Mortal Wkly Rep. 2002; 51:1–44.

Purva M. Hand hygiene: Back to the basics of infection control. Indian J Med Res. 2011;134(5):611–620.

Aiello EA, Larson EL. Causal inference: The case of hygiene and health. American Journal of Infection Control. 2002;30(8): 503-510.

Paul R1, Das NK, Dutta R, Bandyopadhyay R, Banerjee AK. Bacterial contamination of the hands of doctors: A study in the medicine and dermatology wards. Indian J Dermatol Venereol Leprol. 2011;77(3):307-13.

SFHH (Société Française d’Hygiène Hospitalière). Recommandations Hygiène des mains. Hygiènes. 2009; XVII:102.

Stone SP. Hand hygiene: The case for evidence-based education. J R Soc Med. 2001;94:278–81.

Pratt RJ, Pellowe C, Liveday HP, Robinson N, Smith GW, Barrett S. The EPIC project: developing national evidence- based guidelines for preventing healthcare associated infections. J Hosp Infect. 2001;47(Suppl A):S3–82.

Ashurst JV, Nappe TM. Isopropanol Toxicity. Treasure Island (FL): StatPearls Publishing; 2019 Jan-.
Available:https://www.ncbi.nlm.nih.gov/books/NBK493181/

Jammalamadaka D, Raissi S. Ethylene glycol, methanol and isopropyl alcohol intoxication. Am. J. Med. Sci. 2010; 339(3):276-81.

Désy O, Carignan D, de Campos-Lima PO. Short-term immunological effects of non-ethanolic short-chain alcohols. Toxicol. Lett. 2012;210(1):44-52.

Slaughter RJ, Mason RW, Beasley DM, Vale JA, Schep LJ. Isopropanol poisoning. Clin Toxicol (Phila). 2014;52(5):470-8.

Ismail M, Kemegne GA, Njayou FN, Penlap V, Mbacham WF, Sado Kamdem SL. Chemical composition, antibiotic promotion and in vivo

toxicity of Piper nigrum and Syzygium aromaticum essential oil. African Journal of Biochemistry Research. 2017;11(10):58-71.

Shiva Rani SK, Neeti S, Udaysree. Antimicrobial activity of black pepper (Piper nigrum L.). Global Journal of Pharmacology. 2013;7(1):87-90.

Rasheeha N, Iftikhar H, Abdul T, Muhammad T, Moazur R, Sohail H, Shahid M, Abu B, Mazhar I. Antimicrobial activity of the bioactive components of essential oils from Pakistani spices against Salmonella and other multi-drug resistant bacteria. BMC Complementary and Alternative Medicine. 2013;(13):265-270.

Thales LC, De O, Maria das Gracas C, Rodrigo de Araùjo S, Eduardo MR, Roberta HP, Victor Maximiliano RT. Inhibitory activity of Syzygium aromaticum and Cymbopogon citrates (DC) Stapf essential oils against Listeria monocytogenes inoculated in bovine ground meat. Brazilian Journal of Microbiology. 2013;44(2):357-365.

Pavithra VK, Bhagya L. Antibacterial activity of black peper (Piper nigrum Linn.). Indian Journal of Natural Products and Ressources. 2010;1(2):213-215.

Nyegue MA, Ndoyé-Foe F, MC, Riwom Essama S, Hockmeni TC, Etoa FX, et Menut C. Chemical composition of essential oils of Eugenia caryophylla and Mentha sp cf perita and their in vitro antifungal activities on six human pathogenic fungi. African Journal of Traditional, Complementary and Alternative Medicines. 2014;11(6):40-46.

Guide to Local. Production: WHO-recommended Handrub Formulations, Organisation Mondiale de la Santé; 2010.
Available:http://www.who.int/gpsc/5may/Guide_to_Local_Production.pdf

Man A, Serban Gâz A, Delia Mare A, Berta Man L, et al. Effects of low-molecular weight alcohols on bacterial viability. Revista Româna de Medicina de Laborator. 2017;25:4.

Ingólfsson HI, Andersen OS. Alcohol’s Effects on Lipid Bilayer Properties. Biophys J. 2011;101(4):847–55.